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Effect of recombinant human interleukin 2 on neutrophil adherence to endothelial cells in vitro

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Abstract

Human neutrophils were demonstrated to possess interleukin-2 receptor (IL-2R)β andγ chains, notα chain and the binding of IL-2 to the IL-2Rβ chain on neutrophils plays an important regulatory role in neutrophil functions. We have investigated in this study the hypothesis that recombinant human IL-2(rhIL-2) can directly activate human neutrophils and increase their adherence to human umbilical vein endothelial cells (HUVEC). In an in vitro microtiter adherence assay, rhIL-2 significantly stimulated neutrophil adherence to HUVEC in a dose- and time-dependent manner. rhILI-2 concentration at 2000 u/ml and 2 hour incubation gave the best neutrophil stimulation. Treatment of neutrophils with rhIL-2 increased the expression of adhesion molecule CD18. Pretreatment of the stimulated neutrophils with a blocking monoclonal antibody to CD18 decreased but not completely blocked the adherence of neutrophils to HUVEC. These data suggest that rhIL-2 can directly stimulate and increase neutrophil adherence to HUVEC by enhancing the expression of CD18 and possibly other adhesion molecules on neutrophil surface. This may be a critical step in the early stage of the vascular leak syndrome (VLS) associated with high dose IL-2 therapy.

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  1. Department of Medical Physics, Hamilton Regional Cancer Center, 699 Concession Street, L8V 5C2, Hamilton, Ontario, Canada

    Jun Li, Steve Gyorffy, Sharon Lee & Cheuk S. Kwok

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Li, J., Gyorffy, S., Lee, S. et al. Effect of recombinant human interleukin 2 on neutrophil adherence to endothelial cells in vitro. Inflammation 20, 361–372 (1996). https://doi.org/10.1007/BF01486739

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