Skip to main content
SpringerLink
Log in

Macromolecular conformations at the water-air interface: Interactions between alpha and beta conformations of polypeptides

  • Polymer Science
  • Published:
Colloid and Polymer Science Aims and scope Submit manuscript

Abstract

Bidimensional miscibility between alpha and beta conformations of polypeptides was investigated at the water-air interface in the 15°–30°C temperature range. The polypeptides were poly-γ-methyl-L-glutamate (PGMG), poly-γ-benzyl-L-glutamate (PGBG) and poly-γ-benzyl-L-aspartate (PBBA). The polypeptide conformations, alpha or beta, were checked by IR spectroscopy using the MIR technique.

The spreading isotherms for mixed monolayers alpha-PGMG/alpha-PGBG and beta-PGMG/beta-PBBA showed bidimensional miscibility both for alpha-alpha and beta-beta mixtures.

For the alpha-alpha system, attractive interactions among the polypeptide alphahelices were found (ΔGmix<0) and the driving factor appeared to be the entropic one (packing). Compressibility moduli and surface potential measurements showed a “fluidification” effect of alpha-PGBG on mixed monolayers. In the case of beta-beta mixed monolayers, ideal behaviour was observed and no “fluidification” effect detected.

Scanning electron micrographs made on collapsed monolayers showed hexagonal structures for alpha-alpha mixtures and no well-defined or characterized features for the beta-beta system.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Gabrielli G, Puggelli M, Faccioli R (1971) J Colloid Interface Sci 37:213

    Google Scholar 

  2. Gabrielli G, Puggelli M, Faccioli R (1972) J Colloid Interface Sci 41:63

    Article  Google Scholar 

  3. Gabrielli G, Puggelli M, Faccioli R (1973) J Colloid Interface Sci 44:177

    Google Scholar 

  4. Baglioni P, Dei L, Puggelli M (1985) Colloid Polym Sci 263:266

    Google Scholar 

  5. Baglioni P, Dei L, Gabrielli G (1986) Colloid Polym Sci 264:241

    Google Scholar 

  6. Puggelli M, Gabrielli G (1977) J Colloid Interface Sci 61:420

    Google Scholar 

  7. Gabrielli G, Maddii A (1978) J Colloid Interface Sci 64:19

    Article  Google Scholar 

  8. Gabrielli G, Baglioni P, Maddii A (1981) J Colloid Interface Sci 79:268

    Google Scholar 

  9. Gabrielli G, Puggelli M, Baglioni P (1982) J Colloid Interface Sci 86:485

    Article  Google Scholar 

  10. Gabrielli G, Davidson A (1975) Prog Colloid Polym Sci 58:169

    Google Scholar 

  11. Gabrielli G, Puggelli M (1975) Adv Chem Ser “Monolayers” 144:347

    Google Scholar 

  12. Gabrielli G, Ferroni E, Puggelli M, Huggins ML (1978) Colloid Polym Sci 256:417

    Google Scholar 

  13. Baglioni P, Gallori E, Gabrielli G, Ferroni E (1982) J Colloid Interface Sci 88:221 and references therein

    Google Scholar 

  14. Malcolm BR (1973) In: Danielli JF, Rosemberg MD, Cadenhead DA (eds) Progress in Surface and Membrane Science, Vol 7. Academic Press, New York, pp 183–229 and references therein

    Google Scholar 

  15. Miller R (1971) In: Danielli JF, Rosemberg MD, Cadenhead DA (eds) Progress in Surface and Membrane Science, Vol 4. Academic press, New York, pp 299–350 and references therein

    Google Scholar 

  16. Blodgett KB (1935) J Am Soc 57:1997

    Google Scholar 

  17. Blodgett KB, Langmuir J (1944) Phys Rev 51:488

    Google Scholar 

  18. Ries HE Jr, Walker DC (1961) J Colloid Interface Sci 16:361

    Google Scholar 

  19. Ries HRJr, Matsumoto M, Uyeda N, Suito E (1975) Adv Chem Ser “Monolayers” 144:286 and references therein

    Google Scholar 

  20. Bergern JA, Gaines GL Jr (1967) J Colloid Interface Sci 23:292

    Google Scholar 

  21. Jaycock MJ, Parfitt GD (1981) In: Chemistry of Interfaces. Ellis Horwood Ltd, Chichester, p 69

    Google Scholar 

  22. Adamson AW (1982) In: The Physical Chemistry of Surfaces. 4th Ed, John Wiley and Sons, New York, p 144

    Google Scholar 

  23. Gaines GL Jr (1966) In: Insoluble Monolayers at Liquid-Gas Interfaces, Chap 3. Wiley-Interscience, New York

    Google Scholar 

  24. Baglioni P, Dei L, Gabrielli G (1983) J Colloid Interface Sci 93:402

    Google Scholar 

  25. Dei L, Baglioni P, Gabrielli G (1987) In: Bothorel P, Mittal KL (July 1984) (eds) Surfactants in Solution. Proceedings of the 5th International Symposium on Surfactants in Solution, Bordeaux, New York, Vol 5, pp 979–994

  26. Huggins ML (1973) Kolloid ZZ Polym 251:449 and references therein

    Google Scholar 

  27. Gabrielli G, Ferroni E, Puggelli M, Huggins ML (1978) Colloid Polym Sci 256:417

    Google Scholar 

  28. Crisp DJ (1949) In: Surface Chemistry Suppl Research. Butterworth, London, pp 17–23

    Google Scholar 

  29. Gaines GL Jr (1966) In: Insoluble Monolayers at Liquid-Gas Interfaces. Wiley-Interscience, New York, pp 281–300

    Google Scholar 

  30. Goodrich FC (1957) Proceedings 2nd International Congress Surface Activity, Vol 1, p 85

    Google Scholar 

  31. Barnes GT, Bacon KJ, Ash JM (1980) J Colloid Interface Sci 76:263

    Google Scholar 

  32. Davies JT, Rideal EK (1963) In: Interfacial Phenomena. Academic Press, New York and London, p 265

    Google Scholar 

  33. Baglioni P, Carlà M, Dei L, Martini E (1987) J Phys Chem 91:1460

    Google Scholar 

  34. Dei L, Baglioni P, Carlà M, Martini E (1986) In: Mittal KL (ed) Surfactants in Solution. Proceedings of the 6th International Symposium on Surfactants in Solution (August), New Delhy, Plenum Press, in press

    Google Scholar 

  35. Baglioni P, Dei L, Ferroni E, Gabrielli G (1986) J Colloid Interface Sci 109:109

    Google Scholar 

Download references

Author information

Author notes

  1. G. Gabrielli

    Present address: Dipartimento di Chimica, Università degli Studi di Firenze, Firenze, Italy

Authors and Affiliations

  1. Dipartimento di Chimica, Università degli Studi di Firenze, Firenze, Italy

    P. Baglioni, L. Dei, F. M. Innocenti & A. Niccolai

Authors
  1. P. Baglioni
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. L. Dei
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. Gabrielli
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. F. M. Innocenti
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. A. Niccolai
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Baglioni, P., Dei, L., Gabrielli, G. et al. Macromolecular conformations at the water-air interface: Interactions between alpha and beta conformations of polypeptides. Colloid & Polymer Sci 266, 783–792 (1988). https://doi.org/10.1007/BF01417862

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01417862

Key words

Search

Navigation