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Amylase mRNA transcripts in normal tissues and neoplasms: the implication of different expressions of amylase isogenes

  • Original Papers
  • Experimental Oncology
  • Published:
Journal of Cancer Research and Clinical Oncology Aims and scope Submit manuscript

Abstract

To understand the cellular origin and mechanism of gene expression in amylase-producing cancers, the phenotyping of amylase isogenes by the polymerase chain reaction and restriction-fragment-length polymorphism using restriction endonucleasesTaqI,DdeI,HinfI, andAfaI were performed for 3 amylase-producing lung adenocarcinomas, 16 lung cancers without hyperamylasemia, other human malignant neoplasms, cultured cell lines, and normal tissues. In addition, amylase mRNA transcripts were semi-quantified by the limited polymerase chain reaction. Amylase mRNA transcripts were detected in all of the tissues examined. TheAMY1 gene (salivary type) was exclusively and highly expressed in the salivary glands and the amylase-producing lung adenocarcinomas. Coexpression of theAMY1 gene andAMY2 gene (pancreatic type) was observed in most of the lung cancers without hyperamylasemia, lung tissue, and cells scraped from the tracheal epithelium, thyroid, and female genital tract (ovary, fallopian tube, and uterus cervix), while minimal levels of mRNA transcripts of theAMY2 gene were detected in other malignant neoplasms, various normal tissues, and the cultured cell lines. All mRNA transcripts identified as being those of theAMY2 gene were further identified as being from theAMY2B gene except for the transcripts from the pancreas, in which theAMY2A gene andAMY2B gene were coexpressed. On the basis of these results, the clinical occurrence of amylase-producing cancer likely relates to the tissues expressing theAMY1 gene, while theAMY2B gene, which evolutionarily is the oldest gene among human amylase isogenes, is constitutively expressed in various tissues.

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Abbreviations

AMY1 :

salivary-type amylase gene

AMY1A, AMY1B, andAMY1C :

salivary-type amylase isogenes

AMY2 :

pancreatic amylase gene

AMY2A andAMY2B :

pancreatic amylase isogenes

PCR:

polymerase chain reaction

RT:

reverse transcriptase

RFLP:

restriction-fragment-length polymorphism

LTR:

long terminal repeat

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Authors and Affiliations

  1. Department of Respiratory Medicine, Juntendo University, School of Medicine, 2-1-1, Hongo, Bunkyo-ku, Tokyo 113, Japan

    Kuniaki Seyama, Kazuhisa Takahashi, Hideki Takahashi & Shiro Kira

  2. Department of Respiratory Medicine, Division of Cancer Control, Institute of Development Aging, and Cancer, Tohoku University, Seiryomachi 4-1, Aoba-ku, Sendai 980, Japan

    Toshihiro Nukiwa

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  1. Kuniaki Seyama
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  2. Toshihiro Nukiwa
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  3. Kazuhisa Takahashi
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  4. Hideki Takahashi
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  5. Shiro Kira
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Seyama, K., Nukiwa, T., Takahashi, K. et al. Amylase mRNA transcripts in normal tissues and neoplasms: the implication of different expressions of amylase isogenes. J Cancer Res Clin Oncol 120, 213–220 (1994). https://doi.org/10.1007/BF01372559

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  • DOI: https://doi.org/10.1007/BF01372559

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