Abstract
The YTTTC pentanucleotide short tandem repeat polymorphism HumCD4 was studied in an Italian population sample. PCR products were compared to an allelic ladder by manual PAGE and silver staining. A total of 6 alleles ranging from 5 to 12 repeats were represented in the analysed sample, of which 3 alleles (10, 6 and 5 repeats) were predominant and displayed a combined frequency of 0.91. Successful amplification was obtained from different sources such as blood and urine stains, teeth and paraffin embedded tissues. Results were also determined in cases of severely degraded DNA. We consider that the HUMCD4 polymorphism may be a useful tool for individual identification, paternity testing, population studies and have also employed this locus to monitor engraftment of bone marrow transplantation.
References
Bassam BJ, Caetano-Anollés G, Gresshof PM (1991) Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal Biochem 196:80–83
Edwards MC, Clemens PR, Tristan M, Pizzuti A, Gibbs RA (1991) Pentanucleotide repeat length polymorphism at the human CD4 locus. Nucleic Acid Res 19:4791
Evett I, Gill P, Scranage JK (1996) Establishing the robustness of STR statistics for forensic applications. Am J Hum Genet (in press)
Executive Committee of ISFH (1994) DNA recommendations — (1994) 1994 report concerning further recommendations of the DNA Commission of the ISFH regarding PCR-based polymorphisms in STR (Short tandem repeat) systems. Forensic Sci Int 69:103–104
Gill P, Evett I (1995) Population genetics of short tandem repeat (STR) loci. Genetica 96:69–87
Goelz SE, Hamilton SR, Vogelstein B (1985) Purification of DNA from formaldehyde fixed and paraffin embedded human tissue. Biochem Biophys Res Comm 130:118–126
Kimpton CP, Gill P, Walton A, Urquhart A, Millican ES, Adams M (1993) Automated DNA profiling employing multiplex amplification of short tandem repeat loci. PCR Methods Applic 3:13–22
Kurosaky K, Matsushita T, Ueda S (1993) Individual DNA identification from ancient human remains Am J Hum Genet 53:638–643
Nei M (1987) Molecular evolutionary genetics. Columbia University Press, New York
Piazza A, Olivetti E, Barbanti M, Reali G, Domenici R, Giari A, Benciolini P, Caenazzo L, Cortivo P, Bestetti A, Bonavita V, Crinò C, Pascali VL, Fiori A, Bargagna M (1989 a) The distribution of some polymorphisms in Italy. Gene Geography 3:69–139
Piazza A, Olivetti E, Griffo RM, Rendine S, Amoroso A, Barbanti M, Caruso C, Conighi C, Conte R, Favoino B, Galliano L, Luciani G, Magrì D, Martinetti M, Mattuz PL, Menicucci A, Misefari V, Moro L, Purpura M, Savi M (1989 b) The distribution of HLA antigens in Italy. Gene Geography 3:141–164
Smith CBA (1986) Chi-squared tests with small numbers. Ann Hum Genet 50:163–167
Urquhart A, Kimpton CP, Downes TJ, Gill P (1994) Variation in short tandem repeat sequences — a survey of twelve microsatellite loci for use as forensic identification markers. Int J Legal Med 107:13–20
Walter H, Dannewitz A, Eberhardt D, Trautmann M, Pacaci M, Rickards O, De Stefano GF, Biondi G (1989) Serum protein polymorphisms (HP; TF-, GC-, PI-subtypes) in Sardinia. Gene Geography 3:165–171
Yamada Y, Yamamoto K, Yoshii T, Ishiyama I (1989) Analysis of DNA from tooth and application to forensic medicine. Nippon Hoikazu Zasshi 43 (5):420–423
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Casarino, L., Mannucci, A., Bruni, G. et al. Forensic evaluation of HUMCD4: An Italian database. Int J Leg Med 109, 49–51 (1996). https://doi.org/10.1007/BF01369604
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01369604