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Recognition of SV40-VP2 in the infected cell by antipeptide antibodies

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Summary

Antipeptide antibodies were elicited against two synthetic peptides corresponding to amino acids 47–55 and 98–103 of the structural protein VP 2 of SV 40. The induced antibodies proved to be VP 2-specific in an immunoblot. In immunofluorescence these antibodies showed a discrete nuclear and perinuclear staining pattern. In immune electron microscopy studies the induced antibodies did not bind to major virions suggesting that VP 2 is not present at the surface of SV 40 particles.

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Authors and Affiliations

  1. Department of Medical Microbiology and Virology, Ruhr-University Bochum, Bochum, Federal Republic of Germany

    H. J. Streckert & H. Werchau

  2. Department of Pathology, Ruhr-University Bochum, Bochum, Federal Republic of Germany

    H. J. Sommerfeld & K. Morgenroth

Authors
  1. H. J. Streckert
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  2. H. J. Sommerfeld
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  3. K. Morgenroth
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  4. H. Werchau
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Streckert, H.J., Sommerfeld, H.J., Morgenroth, K. et al. Recognition of SV40-VP2 in the infected cell by antipeptide antibodies. Archives of Virology 123, 399–407 (1992). https://doi.org/10.1007/BF01317273

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  • DOI: https://doi.org/10.1007/BF01317273

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