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The nucleotide sequence of the coat protein gene and 3′ untranslated region of papaya ringspot virus type W (Aust)

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Summary

The nucleotide sequence of the 3′ terminal region of the Australian isolate of papaya ringspot virus type W [PRSV-W (Aust)] was determined. An open reading frame (864 bp), encoding the putative coat protein gene, occurs upstream of the putative 3′ untranslated region (206 bp) and poly(A) tail. A 23 amino acid sequence was obtained from N-terminal analysis of the coat protein from purified virions. This sequence has 100% homology with a region of the amino acid sequence inferred from the nucleic acid sequence of the coat protein gene. However, this region is 13 amino acids downstream from the N terminus predicted for two American isolates of PRSV. The coat protein gene of PRSV-W (Aust) was shown to have 96.8% and 96.4% nucleotide sequence similarity with American isolates of PRSV-W and PRSV-P, respectively.

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  1. Centre for Molecular Biotechnology, Queensland University of Technology, Brisbane, Queensland, Australia

    M. Bateson & J. Dale

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  1. M. Bateson
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  2. J. Dale
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Bateson, M., Dale, J. The nucleotide sequence of the coat protein gene and 3′ untranslated region of papaya ringspot virus type W (Aust). Archives of Virology 123, 101–109 (1992). https://doi.org/10.1007/BF01317141

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  • DOI: https://doi.org/10.1007/BF01317141

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