Summary
Influenza virus neuraminidases of the N1 serotype exhibit a complete dependence on Ca++ ions for activity. It is recommended that viral neuraminidase activity routinely be assessed in buffers containing 5mm CaCl2.
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Carroll, S.M., Paulson, J.C. Complete metal ion requirement of influenza virus N1 neuraminidases. Archives of Virology 71, 273–277 (1982). https://doi.org/10.1007/BF01314879
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DOI: https://doi.org/10.1007/BF01314879