Summary
Serum samples collected in 1984 during a malariometric survey of two villages in the East Sepik Province of Papua New Guinea were tested for antibodies to HTLV-I. None of the villagers showed any symptoms suggestive of retrovirus infection. Eighteen of the 186 (9.5%) sera tested at that time were found to be positive. Blood samples were subsequently obtained from fifteen of the eighteen positives and subjected to analysis by enzyme-linked immunosorbent assay (ELISA), radioimmuno assay (RIA), radioimmunoprecipitation assay (RIPA), and Western blot (WB). Fourteen of the fifteen gave a positive ELISA response, but none were unequivocally positive by p 24 RIA. All sera tested were reactive togag antigens by WB, but gave “indeterminate” results currently accepted criteria. Notably absent from the WB profiles of all of the study subjects was an antibody response to HTLV-I envelope protein gp 46. It is possible that these antibody responses are directed against a variant of HTLV-I or to a novel retrovirus which possesses core antigens similar to those of HTLV-I but has different envelope antigens. Until a virus is isolated, or the viral genome is identified in infected lymphocytes, the possibility remains that the response may be due to factors unrelated to retrovirus infection.
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Agius G, Biggar RJ, Alexander SS, Waters DJ, Drummond JE, Murphy EL, Weiss SH, Levine PH, Blattner WA (1988) Human T-lymphotropic virus type I antibody patterns: evidence of difference by age and risk group. J Infect Dis 158: 1235–1244
Ajdukiewicz A, Yanagihara R, Garruto RM, Gajdusek DC, Alexander SS (1989) HTLV-I myeloneuropathy in the Solomon Islands. N Engl J Med 321: 615–616
Alexander SS Jr, Tai CC, Ting RL, Corrigan AE, Bodner AJ, Julien DW (1985) Western blot analysis of human T-cell lymphotropic virus-III proteins using an avidin-biotin system. In: Program and abstracts of the 1st International Conference on Aquired Immunodeficiency Syndrome (AIDS). US Government Printing Office, Atlanta
Asher DM, Goudsmit J, Pomeroy KL, Garruto RM, Bakker M, Ono SG, Elliot N, Harris K, Askins H, Eldadah Z, Goldstein AD, Gajdusek DC (1988) Antibodies to HTLV-I in populations of the Southwestern Pacific. J Med Virol 26: 339–351
Babona DV, Nurse GT (1988) HTLV-I antibodies in Papua New Guinea. Lancet 2: 1148
Biggar RJ (1987) Epidemiology of human retroviruses and related clinical conditions. In: Broder S (ed) AIDS, modern concepts and therapeutic challenges. Marcel Dekker, New York, pp 91–121
Biggar RJ, Gigase PL, Melbye M, Kestens L, Sarin P, Bodner AJ, Demedts P, Stevens WJ, Paluku L, Delacollette C, Blattner WA (1985) ELISA HTLV retrovirus reactivity associated with malaria and immune complexes in healthy Africans. Lancet 2: 520–523
Brindle RJ, Eglin RP, Parsons AJ, Hill AVS, Selkon JB (1988) HTLV-I, HIV, hepatitis B and hepatitis delta in the Pacific and South-East Asia: a serological survey. Epidemiol Infect 100: 153–156
Clark JW, Robert-Guroff M, Ikehara O, Henzan E, Blattner WA (1985) Human T-cell leukaemia-lymphoma virus type I and adult T-cell leukaemia-lymphoma in Okinawa. Cancer Res 45: 2849–2852
Constantine NT, Fox E (1989) Need to confirm HTLV-I screening assays. Lancet 1: 108–109
Currie B, Hinuma Y, Imai J, Cumming S, Doherty R (1989) HTLV-I antibodies in Papua New Guinea. Lancet 1: 1137
Desowitz RS, Spark RA (1987) Malaria in the Maprik area of the Sepik region, Papua New Guinea: 1957–1984. Trans R Soc Trop Med Hyg 81: 175–176
Garruto RM, Slover M, Yanagihara R, Mora C, Alexander SS, Asher DM, Rodgers-Johnson P, Gajdusek DC (1988) High prevalence of human T-lymphotropic virus type I (HTLV-I) infection in isolated populations of the Western Pacific without Japanese or African contact. In: Abstracts of the 37th Annual Meeting of the American Society of Tropical Medicine and Hygiene, Washington, DC, December 4–8, 1988, p 263
Garruto RM, Yanagihara R, Asher DM (1989) Seroepidemiology of human T-lymphotropic virus type I in the Pacific region. In: Zaninovic V (ed) Retrovirus humanos HTLV-I: paraparesia espastica y linfomas. Editorial Fervia, Cali, Colombia, pp 95–108
Gessain A, Barin F, Vernant JC, Gout O, Maurs L, Calender A, de The G (1985) Antibodies to human T-lymphotropic virus type-I in patients with tropical spastic paraparesis. Lancet 2: 407–410
Hattori S, Kiyokawa T, Imagawa K, Shimizu F, Hashimura E, Seiki M, Yoshida M (1984) Identification of gag and env gene products of human T-cell leukemia virus (HTLV). Virology 136: 338–347
Hinuma Y, Komoda H, Chosa T, Kondo T, Kohakura M, Takenaka T, Kikuchi M, Ichimaru M, Yunoki K, Sato I, Matsuo R, Takiuchi Y, Uchino H, Hanaoka M (1982) Antibodies to adult T-cell leukaemia virus-associated antigen (ATLA) in sera from patients with ATL and controls in Japan: a nation-wide sero-epidemiologic study. Int J Cancer 29: 631–635
Hinuma Y, Nagata K, Hanaoka M, Matsumoto T, Kinoshita KI, Shirakawa S, Miyoshi I (1981) Adult T-cell leukaemia: antigen in an ATL cell line and detection of antibodies to the antigen in human sera. Proc Natl Acad Sci USA 78: 6476–6480
Hrdy DB, Carlson JR, Yee JL, Armstrong MYK (1989) Need to confirm HTLV-I screening assays. Lancet 1: 109
Ikeda M, Fujino R, Matsui T, Yoshida T, Komoda H, Imai J (1984) A new agglutination test for serum antibodies to adult T-cell leukaemia virus. Gann 75: 45–848
Kalyanaraman VS, Sarngadharan MG, Bunn PA, Minna JD, Gallo RC (1981) Antibodies in human sera reactive against an internal structural protein of human T-cell lymphoma virus. Nature 294: 271–273
Kanner SB, Cheng-Mayer C, Geffin RB, Parks WP, Beltz GA, Arthur LO, Samuel KP, Papas TS (1986) Human retroviral env and gag polypeptides: serological assays to measure infection. J Immunol 137: 674–678
Kazura JW, Saxinger WC, Wenger J, Forsyth K, Lederman MM, Gillespie JA, Carpenter CCJ, Alpers MP (1987) Epidemiology of human T cell leukaemia virus type I infection in East Sepik Province, Papua New Guinea. J Infect Dis 155: 1100–1107
Koyanagi Y, Hinuma Y, Schneider J, Chosa T, Hunsmann G, Kobayashi N, Hatanaka M, Yamamoto N (1984) Expression of HTLV-specific polypeptides in various human T-cell lines. Med Microbiol Immunol (Berl) 173: 127–140
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T 4. Nature 227: 680–685
Lee TH, Coligan JE, Homma T, McLane MF, Tachibana N, Essex M (1984) Human T-cell leukemia virus associated membrane antigens: identity of the major antigens recognized after virus infection. Proc Natl Acad Sci USA 81: 3856–3860
Popovic M, Reitz MS, Sarngadharan MG, Robert-Guroff M, Kalyanaraman VS, Nakao Y, Miyoshi I, Minowada J, Yoshida M, Ito Y, Gallo RC (1982) The virus of Japanese adult T-cell leukaemia is a member of the human T-cell leukaemia virus group. Nature 300: 63–66
Robert-Guroff M, Schupbach J, Blayney DW, Kalyanaraman VS, Merino F, Sarngadharan MG, Clark JW, Saxinger WC, Blattner WA, Gallo RC (1984) Seroepidemiologic studies on human T-cell leukaemia/lymphoma virus, type I. In: Gallo RC, Essex M, Gross L (eds) Human T-cell leukaemia/lymphoma viruses. Cold Spring Harbor Press, New York, pp 285–295
Saxinger C, Gallo RC (1983) Methods in laboratory investigation. Application of the indirect enzyme-linked immunosorbent assay microtest in the detection and surveillance of human T-cell leukemia-lymphoma virus. Lab Invest 49: 371–377
Schupbach J, Sarngadharan M, Gallo RC (1984) Antigens on HTLV-infected cells recognized by leukaemia and AIDS sera are related to HTLV viral glycoprotein. Science 224: 506–508
Taguchi H, Sawada T, Fujishita M, Morimoto T, Nilya K, Miyoshi I (1983) Enzyme-linked immunosorbent assay of antibodies to adult T-cell leukaemia-associated antigens. Gann 74: 185–187
Towbin H, Staehlin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76: 4350–4354
Weber JN, Banatvala N, Clayden S, McAdam KPWJ, Palmer S, Moulsdale H, Tosswill J, Dilger P, Amann S (1989) HTLV-I infection in Papua New Guinea: evidence for serologic false positivity. J Infect Dis 159: 1025–1029
Yoshida M (1983) Human leukemia virus associated with adult T-cell leukemia. Jpn J Cancer Res 74: 777–789
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Sanders, R.C., Levin, A., Anian, G. et al. HTLV-I antibody studies in villagers in East Sepik Province, Papua New Guinea. Archives of Virology 114, 27–35 (1990). https://doi.org/10.1007/BF01311009
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DOI: https://doi.org/10.1007/BF01311009