Summary
Aujeszky's disease (AD; pseudorabies) viruses isolated in Northern Ireland over a 20 year period were compared with isolates from other parts of the world using restriction endonuclease analysis of virus DNA. When the numbers of Bam H1, Kpn 1 and Sal 1 restriction sites were considered, pathogenic Northern Ireland isolates resembled viruses isolated in England, Hungary and the U.S.A. and could be differentiated from viruses isolated in Denmark, Belgium and the Netherlands. The avirulent Northern Ireland isolate NIA4 and the Bartha vaccine strain were very similar to each other and could be distinguished from pathogenic isolates.
While almost all the pathogenic viruses isolated in Northern Ireland from 1963 to 1983 appeared to possess the same number of restriction sites none of the viruses, even those made at the same farm during one outbreak of infection, were identical. The differences were confined to variation in the sizes of certain fragments which map in “variable” regions of the genome.
Similar content being viewed by others
References
Ben-Porat, T., Deatly, A. M., Easterday, B. C., Galloway, D., Kaplan, A. S., McGregor, S.: Latency in psuedorabies virus. In:Wittmann, G., Gaskell, R. M., Rziha, H.-J. (eds.), Latent Herpesvirus Infections in Veterinary Medicine, 365–383. The Hague: Nijhoff 1984.
Geek, P., Jr., Nagy, E., Lomniczi, B.: Differentiation between Aujeszky's disease virus strains of different virulence by restriction endonuclease analysis of the DNA. Magyor Allator. Lapja37, 651–662 (1982).
Gielkens, A. L. J., Berns, A. J. M.: Differentiation of Aujeszky's disease virus strains by restriction endonuclease analysis of the viral DNA's. Current topics in Vet. Med. Ani. Sci.17, 3–13 (1982).
Herrmann, S.-C., Heppner, B., Ludwig, H.: Pseudorabies viruses from clinical outbreaks and latent infectious grouped into four genome types. In:Wittmann, G., Gaskell, R. M., Rziha, H.-J. (eds.), Latent Herpesvirus Infections in Veterinary Medicine, 387–401. The Hague: Nijhoff 1984.
Lomniczi, B., Blankenship, M. L., Ben-Porat, T.: Deletions in the genomes of pseudorabies virus vaccine strains and existence of four isomers of the genomes. J. Virol.49, 970–979 (1984).
Wathen, M. W., Pirtle, E. C.: Stability of the pseudorabies virus genome afterin vivo serial passage. J. gen. Virol.65, 1401–1404 (1984).
Author information
Authors and Affiliations
Additional information
With 4 Figures
Rights and permissions
About this article
Cite this article
Todd, D., McFerran, J.B. Restriction endonuclease analysis of Aujeszky's disease (pseudorabies) virus DNA: Comparison of Northern Ireland isolates and isolates from other countries. Archives of Virology 86, 167–176 (1985). https://doi.org/10.1007/BF01309822
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01309822