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Cloning of the thymidylate synthetase gene (thyPIG 3) from theBacillus subtilis temperate phage IG 3

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Summary

ThethyPIG 3 gene fromBacillus subtilis bacteriophage IG 3 was cloned in the plasmid pHV 33. Two recombinant plasmids, pISL 61 and pISL 62 carrying that gene are effective in transforming to thymine prototrophy bothEscherichia coli (by complementation) andB. subtilis (by complementation and recombination). The comparison of cloned fragment containing thethyPIG 3 gene and thethyP 3 gene from phage ϕ3 T, by restriction analysis and DNA hybridization, suggests a strong homology between the two. ThethyPIG 3 gene was mapped in this study in the central region of the IG 3 genome.

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Authors and Affiliations

  1. Centro de Tecnologia Quimica e Biológica (CTQB), Oeiras

    Ilda Santos & Herminia de Lencastre

  2. Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Portugal

    Ilda Santos & Herminia de Lencastre

  3. The Rockefeller University, 10021, New York, NY, USA

    Herminia de Lencastre

Authors
  1. Ilda Santos
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  2. Herminia de Lencastre
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Santos, I., de Lencastre, H. Cloning of the thymidylate synthetase gene (thyPIG 3) from theBacillus subtilis temperate phage IG 3. Archives of Virology 127, 65–74 (1992). https://doi.org/10.1007/BF01309575

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  • DOI: https://doi.org/10.1007/BF01309575

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