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Monocyte zinc andIn vitro prostaglandin E2 and interleukin-1β production by cultured peripheral blood monocytes in patients with Crohn's disease

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Abstract

This study investigated the relationship between zinc status and prostaglandin E2 and interleukin-1β production by cultured monocytes in patients with Crohn's disease. Monocyte zinc was significantly decreased in both 12 inpatients and 22 outpatients compared with controls (P<0.001) but lymphocyte and polymorphonuclear cell zinc were normal. When cultured monocytes from 10 outpatients with Crohn's disease were stimulated with lipopolysaccharide, prostaglandin E2 production increased markedly, coupled with a fall in monocyte zinc. In matched controls, prostaglandin E2 production was significantly less and monocyte zinc remained stable. No difference in interleukin-1 release was noted between patients and controls. The addition of prednisolone to cell cultures suppressed prostaglandin E2, interleukin-1 synthesis, and monocyte zinc did not change. Zinc chloride augmented prostaglandin E2 production in patients, but not controls, and interleukin-1 remained stable. These results demonstrate a link between low monocyte zinc concentration and excessive prostaglandin production in patients with Crohn's disease.

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Authors and Affiliations

  1. Department of Medicine, St. James's University Hospital, Clinical Sciences Building, LS9 7TF, Leeds, UK

    Helen F. Goode PhD, B. J. Rathbone MD, J. Kelleher PhD & B. E. Walker MD

  2. Gastroenterology Unit, The General Infirmary, Leeds, UK

    Helen F. Goode PhD, B. J. Rathbone MD, J. Kelleher PhD & B. E. Walker MD

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  1. Helen F. Goode PhD
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  2. B. J. Rathbone MD
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  3. J. Kelleher PhD
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  4. B. E. Walker MD
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Goode, H.F., Rathbone, B.J., Kelleher, J. et al. Monocyte zinc andIn vitro prostaglandin E2 and interleukin-1β production by cultured peripheral blood monocytes in patients with Crohn's disease. Digest Dis Sci 36, 627–633 (1991). https://doi.org/10.1007/BF01297030

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  • DOI: https://doi.org/10.1007/BF01297030

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