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Protective effect of 16,16-dimethyl prostaglandin E2 on isolated rat hepatocytes against complement-mediated immune attack

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Abstract

16,16-Dimethyl prostaglandin E2 was examined for its ability to inhibit complement-mediatedin vitro hepatocytolysis by an antigen-antibody reaction. In the presence of fresh rat serum as a source of complement, 5-min culture of isolated rat hepatocytes with a monoclonal antibody against a rat liver-specific membranous antigen resulted in a marked, significant elevation in lactate dehydrogenase leakage into the culture medium. However, with heat-inactivated rat serum, such a reaction did not occur, indicating that the hepatocytolysis induced by the antibody was attributable to the membrane damaging action of complement activated by an antigen-antibody reaction. Pretreatment of the hepatocyte with 16,16-dimethyl prostaglandin E2 significantly suppressed the cytolytic reaction induced by the antibody in a concentration-dependent manner. These results show that 16,16-dimethyl prostaglandin E2 is capable of protecting isolated rat hepatocytes against the membrane-damaging insult of activated complement.

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Authors and Affiliations

  1. Daiichi Pharmaceutical Co., Ltd., 16-13, Kitakasai 1-Chome, Edogawa-ku, Tokyo 134, Japan

    Yoichi Kurebayashi PhD, Takuya Ikeda MSc & Yuko Honda BC

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  1. Yoichi Kurebayashi PhD
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  2. Takuya Ikeda MSc
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  3. Yuko Honda BC
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Kurebayashi, Y., Ikeda, T. & Honda, Y. Protective effect of 16,16-dimethyl prostaglandin E2 on isolated rat hepatocytes against complement-mediated immune attack. Digest Dis Sci 37, 645–649 (1992). https://doi.org/10.1007/BF01296417

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  • DOI: https://doi.org/10.1007/BF01296417

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