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Alterations in dopamine and serotonin uptake systems in the striatum of the weaver mutant mouse

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Summary

In the striatum of the homozygous weaver mutant mouse (wv/wv), dopamine content, uptake and tyrosine hydroxylase activity are decreased compared to wild-type (+/+) mice. In mice heterozygous for the weaver gene (wv/+), these dopaminergic parameters exhibit only minor reductions compared to +/+ mice. Thewv/wv striatum has recently been shown to have an increase in serotonin content. In the present study, the serotonin uptake system of the weaver striatum was investigated. Synaptosomal uptake of [3H] serotonin was determined in the dorsal portion ofwv/wv and +/+ striatum, and serotonin uptake sites were examined by the binding of [3H] citalopram in the striatum ofwv/wv, wv/+ and +/+ mice. The dopamine uptake system was also investigated in all three genotypes via the binding of [3H] mazindol. Synaptosomal uptake of [3H] serotonin was increased by 79% in the dorsal portion of thewv/wv striatum compared to that seen in the +/+ striatum. The binding of [3H] citalopram was increased by 62% in the dorsolateral and by 111% in the dorsomedial portions of thewv/wv striatum compared to +/+. [3H] Citalopram binding in thewv/+ striatum was also higher than +/+, but this increase did not reach statistical significance. Within thewv/wv striatum, [3H] mazindol binding was almost completely absent (88–89% reduction) in the dorsal portion and severely reduced in the other striatal areas. These data support the notion that the dorsal portion of thewv/wv striatum, which has the severest reduction in dopamine uptake, is hyperinnervated by serotonin fibers.

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Author notes

  1. L. C. Triarhou

    Present address: Department of Pathology and Laboratory Medicine (Neuropathology), Indiana University School of Medicine, Indianapolis, IN, USA

Authors and Affiliations

  1. Department of Psychiatry (Institute of Psychiatric Research), Indiana University School of Medicine, Indianapolis, IN, USA

    J. Norton, B. Ghetti & J. R. Simon

  2. Department of Pathology and Laboratory Medicine (Neuropathology), Indiana University School of Medicine, Indianapolis, IN, USA

    B. Ghetti

  3. Department of Biochemistry and Molecular Biology, Indiana University School of Medicine, Indianapolis, IN, USA

    J. R. Simon

  4. Department of Medical and Molecular Genetics, Indiana University School of Medicine, Indianapolis, IN, USA

    J. Norton & B. Ghetti

  5. Department of Program in Medical Neurobiology, Indiana University School of Medicine, Indianapolis, IN, USA

    E. H. Stotz, B. Ghetti, J. R. Simon & L. C. Triarhou

  6. Laboratorios Almirall, S.A., Research Institute, Barcelona, Spain

    J. M. Palacios

  7. Institut für Pathologie, Universität Basel, Basel, Switzerland

    B. Landwehrmeyer

Authors
  1. E. H. Stotz
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  2. J. M. Palacios
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  3. B. Landwehrmeyer
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  4. J. Norton
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  5. B. Ghetti
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  6. J. R. Simon
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  7. L. C. Triarhou
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Stotz, E.H., Palacios, J.M., Landwehrmeyer, B. et al. Alterations in dopamine and serotonin uptake systems in the striatum of the weaver mutant mouse. J. Neural Transmission 97, 51–64 (1994). https://doi.org/10.1007/BF01277962

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  • DOI: https://doi.org/10.1007/BF01277962

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