Abstract
The rates of phosphatidylcholine biosynthesis in the isolated hamster hearts under ischemic and hypoxic conditions were examined. Global ischemia was produced by perfusion of the heart with a reduced flow, whereas hypoxia was produced by perfusion with a N2-saturated buffer. A 51% reduction in the biosynthesis of phosphatidylcholine was observed in the ischemic heart. The reduction was caused by a severe decrease in ATP level which resulted in a diminished conversion of choline into phosphocholine. A 22% reduction in the biosynthetic rate of phosphatidylcholine was also detected in the hypoxic heart. The reduction was caused by a diminished level of CTP which resulted in a decreased conversion of phosphocholine to CDP-choline. No compensatory mechanism was triggered during ischemia, but the CTP: phosphocholine cytidylyltransferase activity was enhanced in the hypoxic heart. Our results demonstrate the possible rate-limiting role of choline kinase and reconfirm the regulatory role of the cytidylyltransferase in the biosynthesis of phosphatidylcholine. (Mol Cell Biochem116: 53–58, 1992)
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
White DA: The phospholipid composition of mammalian tissues. In: Ansell GB, Hawthorne JN, Dawson RMC (eds) Form and function of phospholipids. Elsevier Scientific Publishing, Amsterdam, 1973, pp 441–483
Cullis PR, Hope MJ: Physical properties and functional roles of lipids in membranes. In: Vance DE, Vance JE (eds) Biochemistry of lipids and membranes. Benjamin Cummings Publishing, Menlo Park, CA, 1985, pp 25–72
Coleman R: Membrane-bound enzymes and membrane ultrastructure. Biochem Biophys Acta 300: 1–30, 1973
Berridge MJ, Irvine RF: Inositol triophosphate, a novel second messenger in cellular signal transduction. Nature (London) 312: 315–321, 1984
Exton JH: Signalling through phosphatidylcholine breakdown. J Biol Chem 265: 1–4, 1990
Dhalla NS, Ziegelhoffer A, Harrow JAC: Regulatory role of membrane systems in heart function. Can J Physiol Pharm 55: 1211–1234, 1977
Vance DE: Phospholipid metabolism in eucaryotes. In: Vance DE, Vance JE (eds) Biochemistry of lipids and membranes. Benjamin Cummings Publishing, Menlo Park, CA, 1985, pp 25–72
Zelinski TA, Savard JD, Man RYK, Choy PC: Phosphatidylcholine biosynthesis in isolated hamster hearts. J Biol Chem 255: 11423–11428, 1980
Vance DE, Choy PC: How is phosphatidylcholine biosynthesis regulated? Trends Biochem Sci 4: 145–148, 1979
Hatch GM, O K-M, Choy PC: Regulation of phosphatidylcholine metabolism in mammalian hearts. Biochem Cell Biol 67: 67–77, 1989
Paddon HB, Vance DE: The relationship between cholinephosphate phosphatase alkaline phosphatase and phosphatidylcholine biosynthesis in HeLa cells. Biochim Biophys Acta 488: 181–189, 1977
Arthur G, Choy PC: Acyl specificity of hamster heart CDPcholine: 1,2-diacylglycerol phosphocholine transferase in phosphatidylcholine biosynthesis. Biochim Biophys Acta 795: 221–229, 1984
Hatch GM, Choy PC: Effect of hypoxia on phosphatidylcholine biolsynthesis in the isolated hamster heart. Biochem J 268: 47–54, 1990
Piper HM, Schwartz P, Hutter J, Spieckermann PG: Energy metabolism and enzyme release of cultured adult rat heart muscle cells during anoxia. J Mol Cell Cardiol 16: 995–1007, 1984
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–275, 1951
Brophy PJ, Choy PC, Toone JR, Vance DE: Choline kinase and ethanolamine kinase are separate soluble enzymes in rat liver. Eur J Biochem 78: 491–495, 1977
Vance DE, Pelech SD, Choy PC: CTP: phosphocholine cytidylyltransferase from rat liver. Methods Enzymol 71: 576–581, 1981
O K-M, Choy PC: Solubilization and partial purification of cholinephosphotransferase in hamster tissues. Lipids 25: 122–124, 1990
Choy PC: Control of phosphatidylcholine biosynthesis in myopathic hamster hearts. J Biol Chem 257: 10928–10933, 1982
Choy PC, Paddon HB, Vance DE: An increase in cytoplasmic CTP accelerates the reaction catalyzed by CTP: phosphocholine cytidylyltransferase in poliovirus-infected HeLa cells. J Biol Chem 255: 1070–1073, 1980
Vance DE: CTP: cholinephopshate cytidylyltransferase. In: Vance DE (ed) Phosphatidylcholine metabolism, CRC Press, Boca Raton, FL. 1989, pp 33–46
Slight R, Kent CR: Regulation of phosphatidylcholine biolsynthesis in mammalian cells. I. Effects of phospholipase C treatment on phosphatidylcholine metabolism in chinese hamster ovary cells and LM mouse fibroblasts. J Biol Chem 258: 824–830, 1983
Choy PC, Vance DE: Lipid requirements for activation of CTP phosphocholine cytidylyltransferase from rat liver. J Biol Chem 253: 5163–5167, 1978
Vance DE, Pelech SL: Enzyme translocation in the regulation of phosphatidylcholine biosynthesis. Trends Biochem Sci 9: 17–20, 1984
Katz AM, Messineo FC: Lipid-membrane interactions and the pathogenesis of ischemic damage in the myocardium. Circ Res 48: 1–16, 1981
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Choy, P.C., Chan, M., Hatch, G. et al. Phosphatidylcholine metabolism in ischemic and hypoxic hearts. Mol Cell Biochem 116, 53–58 (1992). https://doi.org/10.1007/BF01270569
Issue Date:
DOI: https://doi.org/10.1007/BF01270569