Summary
Hexon preparations of type 1, 2, 3, 5, 7, and 14 adenoviruses were treated with a water-soluble carbodiimide to cause the blocking of at least one antigen component. Comparative studies of native and CDI-modified hexons, showed the groupspecific component of all hexons to consist of at least two different components; one being CDI-resistant and the other one CDI-sensitive. In addition, a CD-Iresistant subgroup-specific component was found in type 3, 7, and 14 hexons. Animal immune sera did not always contain antibodies against both of the two group-specific components. CDI-treatment of hexons resulted in an increase in their isoelectric points from 4.9–5.4 to 6.4–7.1, as well as an observed reactivity with agar and some slightly acid proteins. The carbodiimide did not cause the formation of hexon complexes to occur. A new effective method designed to obtain highly purified hexon preparations by means of immunoadsorption is described.
Similar content being viewed by others
References
Banks, P. A., J. A. Kasel, M. Huber, R. H. Alford, andV. Knight: Persistence of neutralizing antibody in adult volunteers immunized with adenovirus soluble antigens. Proc. Soc. exp. Biol. (N.Y.)121, 240–243 (1966).
Catsimpoolas, N.: Microisoelectric focusing in polyacrylamide gel columns. Ann. Biochem.26, 480–482 (1968).
Cornick, G., P. B. Sigler, andH. S. Ginsberg: Characterization of crystals of type 5 adenovirus hexon. J. molec. Biol.57, 397–401 (1971).
Döhner, L.: Untersuchungen zum Nachweis von serologischen Unterschieden zwischen den Gruppenantigenen aus verschiedenen Adenovirustypen. II. Demonstration von Antigendifferenzen mit der KBR in der Isofixationstechnik. Z. Immun.-forsch.136, 451–456 (1968).
Döhner, L.: Untersuchungen zum Nachweis von serologischen Unterschieden zwischen den Gruppenantigenen aus verschiedenen Adenovirustypen. III. Demonstration von Antigendifferenzen mit Hilfe der Untersuchung von Komplement-Antigen-Kurven. Z. Immun.-forsch.138, 128–140 (1969).
Döhner, L., undH. Hudemann: Untersuchungen zur Kristallisation von Hexonen der Adenoviren der Gruppe III. Arch. ges. Virusforsch.38, 279–289 (1972).
Franklin, R. M., U. Pettersson, K. Åkervall, B. Strandberg, andL. Philipson: Structural proteins of adenovirus. V. Size and structure of the adenovirus type 2 hexon. J. molec. Biol.57, 383–395 (1971).
Goodfriend, T. L., L. Levine, andG. D. Fasman: Antibodies to bradykinin and angiotensin: A use of carbodiimides in immunology. Science144, 1344–1346 (1964).
Joniau, M., E. Onkelinx, andR. Lontie: Glutaraldehyde polymerized proteins as immunosorbents. Arch. int. Biochim.76, 182–183 (1968).
Khorana, H. G.: The chemistry of carbodiimides. Chem. Rev.53, 145–166 (1953).
Kjellen, L., andH. G. Pereira: Role of adenovirus antigens in the induction of virus neutralizing antibody. J. gen. Virol.2, 177–185 (1968).
Maizel, J. V., Jr., D. O. White, andM. D. Scharff: The polypeptides of adenovirus. I. Evidence for multiple protein components in the virion and a comparison of types 2, 7A, and 12. Virology36, 115–125 (1968a).
Maizel, J. V., Jr., D. O. White, andM. D. Scharff: The polypeptides of adenovirus. II. Soluble proteins, cores, top components and the structure of the virion. Virology36, 126–136 (1968b).
Mancini, G., A. O. Carbonara, andJ. F. Heremans: Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry2, 235–254 (1965).
Martin, R. G., andB. N. Ames: A method for determining the sedimentation behaviour of enzymes: Application to protein mixtures. J. biol. Chem.236, 1372–1379 (1961).
Nasz, I., A. Lengyel, andI. Cserba: Comparative studies of adenovirus hexon antigens. Arch. ges. Virusforsch.36, 80–92 (1972).
Norrby, E.: Biological significance of structural adenovirus components. Curr. Top. Microbiol.43, 1–43 (1968).
Norrby, E.: The relationship between the soluble antigens and the virion of adenovirus type 3. IV. Immunological complexity of soluble components. Virology37, 565–576 (1969).
Pettersson, U.: Structural proteins of adenoviruses. VI. On the antigenic determinants of the hexon. Virology43, 123–136 (1971).
Rosen, L.: A hemagglutination-inhibition technique for typing adenoviruses. Amer. J. Hyg.71, 120–128 (1960).
Sheehan, J. C., P. A. Cruickshank, andG. L. Boshart: A convenient synthesis of water-soluble carbodiimides. J. org. Chem.26, 2525–2528 (1961).
Sheehan, J. C., andP. Hess: A new method of forming peptide bonds. J. Amer. chem. Soc.77, 1067–1069 (1955).
Valentine, R. C., andH. G. Pereira: Antigens and structure of adenovirus. J. molec. Biol.13, 13–20 (1965).
Wilcox, W. C., andH. S. Ginsberg: Production of specific neutralizing antibody with soluble antigens of type 5 adenovirus. Proc. Soc. exp. Biol. (N.Y.)114, 37–42 (1963).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Döhner, L., Dieckmann, U. & Struy, H. Studies on group-specific determinants of adenovirus hexons. Archiv f Virusforschung 42, 254–263 (1973). https://doi.org/10.1007/BF01265650
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01265650