Abstract
Allele and genotype frequencies for 3 tetrameric short tandem repeat loci VWA, HUMTHO1, and F13A1 were determined in a Swiss population sample using multiplex PCR and subsequent electrophoresis in DNA sequencing gels processed by automated laser fluorescence detection. The technique allows single base pair resolution and rapid typing, with a concomitant reduction in the potential for human transcriptional typing errors. All loci meet Hardy-Weinberg expectations. In addition, there is little evidence for association of alleles among the 3 loci. The allelic frequency data can be used in forensic analyses and paternity tests to estimate the frequency of a multiple STR locus DNA profile in the Swiss population.
Zusammenfassung
An einer Schweizer Populationsstichprobe wurden mittels Multiplex-PCR und automatisierter Fluoreszenzdetektion (Applied Biosystem 373A DNA Sequencer) die Allel- und Genotypfrequenzen der Short tandem repeat (STR) loci VWA, HUMTHO1 und F13A1 bestimmt. Die Daten wurden umfangreichen statistischen Analysen unterzogen. Alle Loci erfüllen die Hardy-Weinberg-Kriterien. Die ermittelten Daten erlauben die Abschätzung der Häufigkeit des Vorkommens eines Multiplex-STR-Profils in der Schweizer Bevölkerung zur Anwendung in forensischen DNA-Analysen und Vaterschaftsuntersuchungen.
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Hochmeister, M.N., Jung, J.M., Budowle, B. et al. Swiss population data on three tetrameric short tandem repeat loci — VWA, HUMTH01, and F13A1 —derived using multiplex PCR and laser fluorescence detection. Int J Leg Med 107, 34–36 (1994). https://doi.org/10.1007/BF01247272
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DOI: https://doi.org/10.1007/BF01247272
Key words
- PCR
- Multiplex
- VWA
- HUMTHO1 F13A1
- Fluorescence detection
- Hardy-Weinberg expectations
- Short tandem repeat loci