Summary
The infectivity of FMDV preparations containing growth medium and cellular products was many times more resistant to inactivation by ionizing radiation than was pure virus. About 4×106 rads were required to reach a survival of less than 10−8 in experiments permitting only the direct absorption of radiation energy by the virus preparation. The infectivity of pure virus preparations was shielded from radiation damage by cysteine and gelatin to a lesser extent than by growth medium and cellular products. Radio-sensitivity was not increased by the addition of a sulfhydril binding reagent, p-hydroxymercuribenzoate. Ribonucleic acid extracted from irradiated virus showed the same rate of infectivity loss as did the virus. The infectivity of free RNA, however, was more sensitive to irradiation than intact virus. Ribonucleic acid in the presence of gelatin had the same stability as intact pure virus. Absorbance-temperature measurements showed that loss of infectivity by pure virus was accompanied by degradation of the virus particles, loss of aromaticity in the purine and pyrimidine rings, as well as hydrolysis of the RNA backbone chain.
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Polatnick, J., Bachrach, H.L. Ionizing irradiation of foot-and-mouth disease virus and its ribonucleic acid. Archiv f Virusforschung 23, 96–104 (1968). https://doi.org/10.1007/BF01242118
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DOI: https://doi.org/10.1007/BF01242118