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Quantitative transformation of primate cells by PARA (defective SV40)-adenovirus type 7

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Summary

A quantitativein vitro assay for the transformation of primate cells by PARA (defective SV 40)-adenovirus type 7 has been developed which allows comparison of the suceptibility of primate and rodent cells to PARA transformation. Transformation by PARA followed one-hit kinetics with a ratio of 12,500 plaque forming units of virus for each focus forming unit in the most efficient primate system tested. The ratio of plaque forming units to focus forming units in hamster embryo fibroblasts using the same virus stock was 2500∶1. The ability to quantitatively determine the transforming capacity of PARA depended upon the strain of PARA-adenovirus 7 employed and the cell type transformed. Single foci were isolated and characterized with respect to morphology and antigenicity. All were composed of cells with a morphology consistent with SV 40 transformation and contained SV 40-induced antigenic determinants. None contained detectable adeno virus specific antigens. Transformation did not alter the characteristic of the cells with respect to the ability to support the replication of various viruses.

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Author notes

  1. Fred Rapp

    Present address: Department of Microbiology, College of Medicine, The Milton S. Hershey Medical Center of The Pennsylvania State University, Hershey, Pennsylvania, USA

Authors and Affiliations

  1. Department of Microbiology, College of Medicine, The Milton S. Hershey Medical Center of The Pennsylvania State University, Hershey, Pennsylvania, USA

    Sally S. Layne & Ronald Duff

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  1. Sally S. Layne
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  2. Ronald Duff
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  3. Fred Rapp
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Layne, S.S., Duff, R. & Rapp, F. Quantitative transformation of primate cells by PARA (defective SV40)-adenovirus type 7. Archiv f Virusforschung 39, 74–82 (1972). https://doi.org/10.1007/BF01241530

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  • DOI: https://doi.org/10.1007/BF01241530

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