Summary
Methylene Blue, which is known to oxidise NADPH in red blood cells, was used to assess a possible role of NADPH in the glucose-stimulated secretion of insulin. When islets from rats were incubated with 3 mg/ml of glucose, Methylene Blue (0.5, 1.0, 2.0 or 5.0 μg/ml) significantly decreased the concentration of NADPH, increased that of NADP+ and decreased the NADPH/NADP+ ratio in a dose-dependent manner. This effect was associated with inhibition of the glucose-induced insulin release. No significant change of NADH, NAD+ and ATP could be observed. It is suggested that the secretory response of the pancreatic islet to glucose stimulation depends on the NADPH/NADP+ ratio.
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Montague, W., Taylor, K. W.: Pentitols and insulin release by isolated rat islets of Langerhans. Biochem. J.109, 333–339 (1968)
Heinze, E., Steinke, J.: Glucose metabolism of isolated pancreatic islets: Difference between fetal, newborn and adult rats. Endocrinology88, 1259–1263 (1971)
Ammon, H. P. T.: Der Einfluß des Alters auf Glucoseoxidation and Pyridinnukleotide der Langerhansschen Insel. Aktuel. Gerontol.4, 755–757 (1974)
Ammon, H. P. T., Verspohl, E.: Pyridine nucleotides in pancreatic islets during inhibition of insulin release by exogenous insulin. Endocrinology99, 1469–1476 (1976)
Ammon, H. P. T., Steinke, J.: 6-Aminonicotinamide (6-AN) as a diabetogenic agent. In vitro studies in rats. Diabetes21, 143–148 (1972)
Ammon, H. P. T., Patel, T. N., Steinke, J.: The role of the pentose phosphate shunt in glucose-induced insulin release: In vitro studies with 6-aminomcotinamide, Methylene Blue, NAD+, NADH, NADP+, NADPH and nicotinamide on isolated pancreatic rat islets. Biochim. Biophys. Acta297, 352–367 (1973)
Malaisse, W. J.: Etude de la secretion insulinique in vitro. C. Effets des inhibiteurs du metabolisme glucidique. pp. 53–57. Bruxelles: Arscia 1968
Hellman, B., Idahl, L.-A., Lernmark, A., Sehlin, J., Täljedal, I.-B.: Membrane sulphydryl groups and the pancreatic beta cell recognition of insulin secretagogues. 8th Congress Internat. Diabetes Federation. Excerpta Med. Int. Congr. Series280, 65–78 (1973)
Hasart, E., Jacobasch, G., Rapoport, S.: Changes of NAD(P) level in human erythrocytes under varying pH and under the influence of Methylene Blue. Acta Biol. Med. Germ.28, 603–613 (1972)
Lacy, P. E., Kostianovsky, H.: Methods of isolation of intact islets of Langerhans from the rat pancreas. Diabetes16, 35–41 (1967)
Krebs, H. A., Henseleit, K.: Untersuchungen über die Harnstoffbildung im Tierkörper. Hoppe Seylers Z. Physiol. Chem.210, 33–66 (1932)
Passoneau, J. V., Lowry, O. H.: In: Methoden der enzymatischen Analyse. Bergmeyer, H. U. (Ed.), pp. 2108. Weinheim: Verlag Chemie 1974
Matschinsky, F. M.: Recent advances in quantitative histo- and cytochemistry. Adv. Clin. Biochem.3, 143 (1970)
Henning, H.-J., Wartmann, R.: Auswertung spärlicher Versuchsdaten im Wahrscheinlichkeitsnetz. Ärztl. Forsch.12, 60–66 (1958)
Ammon, H. P. T., Akhtar, M. S., Niklas, H., Hegner, D.: Inhibition of p-chloromercuribenzoate-and glucose-induced insulin release in vitro by Methylene Blue, diamide and tertbutyl hydroperoxide. Mol. Pharmacol.13, 598–605 (1977)
Verspohl, E. J., Händel, M., Ammon, H. P. T.: Pentose-phosphate shunt activity of the rat pancreatic islet: Its dependence on glucose concentration. Endocrinology (in press)
Ammon, H. P. T., Grimm, A., Wagner-Teschner, D., Verspohl, E. J., Händel, M.: GSH/GSSG and NADPH/NADP ratios of pancreatic islets depend on glucose concentration. Diabetes27, 142 (1978) (Abstract)
Ammon, H. P. T., Akhtar, M. S., Grimm, A., Niklas, H.: Effect of Methylene Blue and thiol oxidants on pancreatic islet GSH/GSSG ratios and tolbutamide mediated insulin release in vitro. Naunyn Schmiedebergs Arch. Pharmacol. (in press)
Ammon, H. P. T.: Effect of tolbutamide on aminophylline-, 3,5-AMP-dibutyrate- or glucagon-induced insulin release from pancreatic islets after impairment of pyridine nucleotide metabolism caused by 6-aminonicotinamide (6-AN). Naunyn Schmiedebergs Arch. Pharmacol.290, 251–264 (1975)
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Ammon, H.P.T., Verspohl, E.J. Effect of Methylene Blue on pyridine nucleotides and insulin secretion of rat pancreatic islets. Diabetologia 17, 41–44 (1979). https://doi.org/10.1007/BF01222976
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DOI: https://doi.org/10.1007/BF01222976