Abstract
ATP receptors, activation of which leads to an increase in membrane conductance for monovalent cations and to the appearance of an inward ionic current at negative membrane potential values, have been found on the outer surface of the somatic membrane of sensory ganglionic neurons of rats and cats. The ATP-activated ionic channel is almost equally permeable for ions of the alkali metals and possesses marked permeability even for Tris and tetraethylammonium ions. Simultaneously with this, the channel demonstrates marked anomalous rectification. For the receptor to be activated, one ATP molecule must interact with it. Activation of the receptors is not connected with the use of energy of high-energy bonds. Affinity of the receptors for agonists is increased with a fall of membrane potential. Adenine nucleotides containing at least two phosphoric acid residues are agonists. The most active agonist is ATP. Adenosine monophosphate, adenosine, and puromycin competitively block these receptors. Pyrimidine nucleotides, and also purine nucleotides — guanosine triphosphate and inosine triphosphate — do not interact with ATP receptors. ATP-activated currents develop quickly and are then slowly desensitized. The desensitization time constant decreases with an increase in ATP concentration, to reach about 2 sec at a saturating concentration.
Similar content being viewed by others
Literature cited
I. A. Vladimirova and M. F. Shuba, "Effect of strychnine, hydrastine, and apamin on synaptic transmission in smooth-muscle cells," Neirofiziologiya,10, No. 3, 295 (1978).
O. A. Kryshtal' and V. I. Pidoplichko, "A proton receptor in the nerve cell membrane," Dokl. Akad. Nauk SSSR,255, No. 6, 1494 (1980).
S M. Tishkin, L. V. Baidan, and M. F. Shuba, "The ionic mechanism of the excitatory action of ATP and noradrenalin on smooth-muscle cells," Fiziol. Zh. (Kiev),27, No. 4, 521 (1981).
C. M. Armstrong, "Time course of TEA+-induced anomalous rectification in squid giant axon," J. Gen. Physiol.,50, No. 2, 491 (1966).
B. E. C. Banks, C. Brown, G. M. Burgess, et al., "Apamin blocks certain neurotransmitter-induced increases in potassium permeability," Nature,282, No. 3, 415 (1979).
T. Bleehen, "The effects of adenine nucleotides on cutaneous afferent nerve activity," Br. J. Pharmacol.,62, No. 4, 573 (1978).
T. Bleehen and C. A. Keele, "Observation on the algogenic actions of adenosine compounds on the human blister base preparation," Pain,3, No. 2, 367 (1977).
G. Burnstock, "A basis for distinguishing two types of purinergic receptors," in: Cell Membrane Receptors for Drugs and Hormones: A Multidisciplinary Approach, Raven Press, New York (1978), pp. 107–118.
R. A. Davidoff, "Gamma-aminobutyric acid antagonism and presynaptic inhibition in the frog spinal cord," Science,175, 331 (1972).
D. V. Gallacher, "Are there purinergic receptors on parotid acinar cell," Nature,296, No. 1, 83 (1982).
W. C. de Groat, "GABA-depolarization of a sensory ganglion: antagonism by picrotoxin and bicucullin," Brain Res.,38, No. 2, 429 (1972).
W. Feldberg and C. O. Hebb, "The stimulating action of phosphate compounds on the perfused superior cervical ganglion of the cat," J. Physiol. (London),107, No. 2, 210 (1948).
B. Hille, "Ionic selectivity of Na and K channels of nerve membranes," in: Membranes: A Series of Advances, Marcel Dekker, New York (1975), Vol. 3, pp. 255–323.
B. Hille and W. Schwarz, "Potassium channels as multi-ion singlefile pores," J. Gen. Physiol.,72, No. 4, 409 (1978).
B. Katz, "Les constantes électriques de la membrane du muscle," Arch. Sci. Physiol.,2, No. 2, 285 (1949).
P. G. Kostyuk, O. A. Kryshtal' (O. A. Krishtal), and V. I. Pidoplichko, "Intracellular perfusion," J. Neurosci., Meth.,4, No. 3, 201 (1981).
J. W. Phillis and P. H. Wu, "The role of adenosine and its nucleotides in central synaptic transmission," Prog. Neurobiol.,16, 187 (1981).
H. B. Stoner and H. M. Green, "Experimental limb ischaemia in man with especial reference to the role of adenosine triphosphate," Clin. Sci.,5, No. 1, 159 (1945).
T. Tomita and H. Watanabe, "A comparison of the effects of adenosine triphosphate with noradrenaline and with the inhibitory potential of the guinea pig Taenia coli," J. Physiol. (London),231, No. 1, 167 (1973).
R. G. Young, "ATP analogs," Adv. Enzymol.,43, 1 (1975).
Additional information
A. A. Bogomolets Institute of Physiology, Academy of Sciences of the Ukrainian SSR, Kiev. Translated from Neirofiziologiya, Vol. 16, No. 3, pp. 327–336, May–June, 1984.
Rights and permissions
About this article
Cite this article
Kryshtal', O.A., Marchenko, S.M. ATP-activated ionic conductance in the somatic membrane of mammalian sensory ganglionic neurons. Neurophysiology 16, 255–263 (1984). https://doi.org/10.1007/BF01065377
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01065377