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Delayed-rectifying potassium channels in mouse peritoneal macrophages: Pharmacological analysis

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Abstract

The effects of 4-aminopyridine, verapamil, and 4-bromophenacylbromide (4-BPB) on the kinetics of delayed outward-rectifying potassium currents (I K) were investigated in cultured mouse peritoneal macrophages using a classical whole-cell patch-clamp technique. The outwardI K was completely blocked by 4-aminopyridine at 1.0 mM concentration. Verapamil at the same concentration also blockedI K completely. Lower concentration (50 µM) of verapamil demonstrated only partial blocking action, which was almost fully reversible, and markedly increased the rate ofI K inactivation. The main effect of 4-BPB on the outwardI K was a significant acceleration ofI K activation and inactivation kinetics. It is suggested that this modulation results from a direct effect of 4-BPB on potassium channels or relates to the arachidonic acid cascade.

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Authors and Affiliations

  1. Saint Petersburg State University, Russia

    Z. I. Krutetskaya, O. E. Lebedev & N. G. Roshchina

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  1. Z. I. Krutetskaya
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  2. O. E. Lebedev
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  3. N. G. Roshchina
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Neirofiziologiya/Neurophysiology, Vol. 26, No. 1, pp. 49–53, January–February, 1994.

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Krutetskaya, Z.I., Lebedev, O.E. & Roshchina, N.G. Delayed-rectifying potassium channels in mouse peritoneal macrophages: Pharmacological analysis. Neurophysiology 26, 40–44 (1994). https://doi.org/10.1007/BF01059992

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  • DOI: https://doi.org/10.1007/BF01059992

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