Summary
Plasma protein fraction from spent effluent in downstream processing was precipitated by zinc chloride. The protein was formulated and reused as a supplement in tissue culture medium. Cell growth and effective product expression were demonstrated in a continuous culture system.
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Ng, P.K., Figueroa, C. & Mitra, G. Plasma protein recovery from spent tissue culture fluid. Biotechnol Lett 13, 261–264 (1991). https://doi.org/10.1007/BF01041481
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DOI: https://doi.org/10.1007/BF01041481