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A thermostable endo-1,4-/B-glucanase from Myceliophthora thermophila

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Summary

Endo-1,4-/B-glucanase from a thermophilic fungusMyceliophthora thermophila has been isolated and purified to homogeneity. The molecular weight of the enzyme was 52,000 with a pI of 4.7, pH-optimum 5.0–6.0, and specific activity 61 IU/mg (40°, pH 5.0); this activity is 2.4 times higher than that of the enzyme fromTrichoderma reesei. The new endoglucanase is very thermostable; its half-life at 65°C is 170 hours, which is 300 times higher than that of the enzyme fromT. reesei.

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Authors and Affiliations

  1. The Carbohydrates Research Laboratory, A.N. Bach Institute of Biochemistry, USSR Academy of Sciences, 117071, Moscow, USSR

    Anatole A. Klyosov & Olga V. Ermolova

  2. Department of Chemistry, Moscow State University, 117234, Moscow, USSR

    Vladimir M. Chernoglazov

Authors
  1. Anatole A. Klyosov
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  2. Olga V. Ermolova
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  3. Vladimir M. Chernoglazov
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Klyosov, A.A., Ermolova, O.V. & Chernoglazov, V.M. A thermostable endo-1,4-/B-glucanase from Myceliophthora thermophila. Biotechnol Lett 10, 351–354 (1988). https://doi.org/10.1007/BF01026164

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