Summary
Fed-batch fermentation of non-supplemented concentrated whey permeate resulted in high ethanol productivity for feeds of lactose for which batch fermentation had a poor performance. At an initial lactose concentration of 100 g/L and a constant lactose feeding rate of 18 g/h we have obtained: ethanol concentration 64 g/L, ethanol productivity 3.3 g/Lh, lactose consumption 100%, ethanol yield 0.47 g/g, and biomass yield 0.058 g/g.
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Abbreviations
- St:
-
total lactose fed per medium volume in the bioreactor, g/L
- Si:
-
initial lactose concentration, g/L
- F:
-
lactpse feeding rate, g/h
- P:
-
final ethanol concentration, g/L
- Yp/s :
-
ethanol yield, g ethanol/g lactose
- Yx/s :
-
biomass yield, g biomass/g lactose
- XS :
-
lactose consumption, %
- Qp:
-
overall ethanol volumetric productivity, g/Lh
- μm:
-
maximum specific growth rate, h
- qsm:
-
maximum specific lactose consumption rate, g/gh
- qpm:
-
maximum specific ethanol production rate, g/gh
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Ferrari, M.D., Loperena, L. & Varela, H. Ethanol production from concentrated whey permeate using a fed-batch culture ofKluyveromyces fragilis . Biotechnol Lett 16, 205–210 (1994). https://doi.org/10.1007/BF01021672
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DOI: https://doi.org/10.1007/BF01021672