Synopsis
The enzymic reduction of folate in the histochemical method described previously by the authors for localizing dihydrofolate dehydrogenase was followed spectrophotometrically by determining the decrease in the folate concentration in the medium during the incubation. In addition the different histochemical localizations of dihydrofolate dehydrogenase and NADH2 dehydrogenase were compared in smears of normal and leukaemic human blood.
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References
Diculescu, I. &Onicescu, D. (1965). La démonstration histochimique des epimérases de l'acide uridine diphospho-d-glucorunique.Histochemie 5, 450–2.
Diculescu, I. &Onicescu, D. (1966). The histochemistry of epimerases. I. Uridine diphosphate glucuronic acid epimerase.Acta histochem. 25, 242–50.
Gerzeli, D. &De Piceis Polver, P. (1969). Un nuovo metodo citochimico per lo studio dell'attivita tetraidrofolico—reduttasica inPlasmodium berghei: analisi dell'effetto di inibitori specifici.Riv. Parassitol. 30, 19–26.
Gerzeli, D. &De Piceis Polver, P. (1970). Studio citochimico della tetraidrofolico deidrogenasi nel sangue di pollo nel corso dell'infezione sperimentale conPlasmodium gallinaceum.Riv. Istochim. morn. pat. 16, 129–37.
Grignani, F., Martelli, M., Tonato, M. &Colonna, A. (1965). Studies on the tetrahydrofolic acid dependent enzyme system in human leukocytes.Acta haemat. 34, 84–7.
Grignani, F., Martelli, M. &Tonato, M. (1967). Dihydrofolic reductase in human blood cells. II. Enzyme activity after amethopterin and actinomycin C administration.Enzymol. biol. clin. 8, 363–78.
Gunlack, B. G., Neal, G. E. &Williams, D. C. (1968). A colorimetric assay system for tetrahydrofolate dehydrogenase.Biochem. Pharmacol. 17, 484–7.
Huennekens, F. M. (1963). The role of dihydrofolic reductase in the metabolism of one carbon unit.Biochemistry (N.Y.) 2, 151–9.
Hutchings, B. L., Stockstad, E. L. R., Boothe, J. H., Mowat, J. H., Waller, C. W., Angier, R. B., Semb, J. &Subbarow, J. (1947). A chemical method for the determination of pteroyglutamic acid and related compounds.J. biol. Chem. 168, 705–8.
Martelli, M., Tonato, M. &Grignani, F. (1967). Dihydrofolic reductase in human blood cells. I. Technique for the study of enzyme activity and intracellular amethopterin content.Enzymol. biol. clin. 8, 353–62.
Onicescu, D., Tasca, L. &Trutza, E. (1970). A histochemical method for dihydrofolate reductase.Histochem. J. 2, 289–95.
Pearse, A. G. E. (1960).Histochemistry. Theoretical and applied. 2nd Ed., p. 909. London: Churchill.
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Onicescu, D., Popescu, M., Tasca, L. et al. On the specificity of a histochemical method for localizing dihydrofolate dehydrogenase. Histochem J 4, 87–90 (1972). https://doi.org/10.1007/BF01004967
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DOI: https://doi.org/10.1007/BF01004967