Abstract
The level of topoisomerase I mRNA was measured in cells of two mouse lymphoma (LY) sublines treated with db-cAMP. A transient increase of the level was observed to be of about 60% of the basic level and to have maximum after the 3 h treatment of LY-S cells. The increase in LY-R subline was two-fold lower. The activity of PKA in a cytosol fraction of LY-S cells was 1.75 times higher than that in LY-R cells. The activity of PKA in membranes and nuclear fraction did not differ significantly in both cell types. When the activity of PKA in LY-S cells was inhibited with H8, no increase of the level of topoisomerase I mRNA was observed upon db-cAMP treatment of cells. We suggest that the activity of PKA in the cytosol controls the expression of topoisomerase I gene in LY cells at high concentration of cAMP.
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Abbreviations
- db-cAMP:
-
dibutyryl-cAMP
- H8:
-
N-[2-(methylamino)ethyl]-5-isoquinolinesulfonamide
- LY:
-
mouse L5178Y lymphoma
- PKA:
-
protein kinase A
- topo I:
-
topoisomerase I
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Czerwinski, R.M., Nieznanski, K., Sochanowicz, B. et al. PKA controls a level of topoisomerase I mRNA in mouse L5178Y lymphoma cells treated with db-cAMP. Mol Biol Rep 19, 93–97 (1994). https://doi.org/10.1007/BF00997153
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DOI: https://doi.org/10.1007/BF00997153