Abstract
DSP4 (N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine) treatment (50 mg/kg i.p., 10 days previously) significantly decreased the noradrenaline (NA) content of the rostral part of the nucleus accumbens. The medial and caudal areas were not affected. The nucleus accumbens appears to receive noradrenergic innervation predominantly from subcoeruleus nuclei of the pons-medulla while the locus coeruleus neurons project to the rostral area. The isoproterenol-induced enhancement of the K+-evoked release of [3H]dopamine (DA) was not affected by DSP4 treatment. Noradrenergic denervation does not appear to have been sufficient to cause up-regulation of postsynaptic β-adrenoceptors.
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Nurse, B., Russell, V. A., and Taljaard, J. J. F. 1984. α2 and β-adrenoceptor agonists modulate [3H]dopamine release from rat nucleus accumbens slices: Implications for research into depression. Neurochem. Res. 9:1231–1238.
Nurse, B., Russell, V. A., and Taljaard, J. J. F. 1985. Effect of chronic desipramine treatment on adrenoceptor modulation of [3H]dopamine release from rat nucleus accumbens slices. Brain Res. 334:235–242.
Plaznik, A., Danysz, W., and Kostowski, W. 1985. Mesolimbic noradrenaline but not dopamine is responsible for organization of rat behaviour in the forced swim test and an anti-immobilizing effect of desipramine. Pol. J. Pharmacol. 37:347–357.
Pellegrino, L. J., Pellegrino, A. S., and Cushman, A. J. 1979. A Stereotoxic Atlas of the Rat Brain pages 10–19. Plenum Press, New York.
Russell, V. A., Lamm, M. C. L., de Villiers, A. S., Taljaard, J. J. F., and Chalton, D. O. 1985. Effects of combined administration ofl-tryptophan and tricyclic antidepressants on α2- and β-adrenoceptors and monoamine levels in rat brain. Neurochem. Res. 10:1661–1671.
McKay, L., Bradberry, C., and Oke, A. 1984. Ascorbic acid oxidase speeds up analysis for catecholamines, indoleamines and their metabolites in brain tissue using high-performance liquid chromatography with electrochemical detection. J. Chromatog. 311:167–169.
Allin, R., Russell, V. A., Lamm, M. C. L., and Taljaard, J. J. F. 1988. Regional distribution of monoamines in the nucleus accumbens of the rat. Neurochem. Res. 13:937–942.
Hetey, L., and Zimmerman, B. 1986. Characterization of the synaptosomal high-affinity uptake of noradrenaline in the nucleus accumbens of rats. Biomed. Biochim. Acta 45:1203–1207.
De France, J. F., Sikes, R. W., and Gottesfeld, Z. 1983. Regional distribution of catecholamines in nucleus accumbens of the rabbit. J. Neurochem. 40:291–293.
O'Donohue, T. L., Crowley, W. R., and Jacobowitz, D. M. 1979. Biochemical Mapping of the noradrenergic ventral bundle projection sites: Evidence for a noradrenergic-dopaminergic interaction. Brain Res. 172:87–100.
Jonsson, G., Hallman, H., Ponzio, F., and Ross, S. 1981. DSP4 (N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine)—a useful denervation tool for central and peripheral noradrenaline neurons. Eur. J. Pharmacol. 72:173–188.
Lookingland, K. J., Chapin, D. S., McKay, D. W., and Moore, K. E. 1986. Comparative effects of the neurotoxins N-chloroethyl-N-ethyl-2-bromobenzylamine hydrochloride (DSP4) and 6-hydroxydopamine on hypothalamic noradrenergic, dopaminergic and 5-hydroxytryptaminergic neurons in the male rat. Brain Res. 365:228–234.
Logue, M. P., Growdon, J. H., Coviella, I. L. G., and Wurtman, R. J. 1985. Differential effects of DSP-4 administration on regional brain norepinephrine turnover in rats. Life Sci. 37:403–409.
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Russell, V.A., Lamm, M.C.L., Allin, R. et al. Effect of selective noradrenergic denervation on noradrenaline content and [3H]dopamine release in rat nucleus accumbens slices. Neurochem Res 14, 169–172 (1989). https://doi.org/10.1007/BF00969634
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DOI: https://doi.org/10.1007/BF00969634