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Monoclonal antibodies as neural cell surface markers

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Abstract

A comparison is made of the immunohistochemistry at the ultrastructural level of three monoclonal antibodies directed against surface components of CNS cells. Hybridomas secreting these antibodies were obtained from two cell fusions of a rat myeloma cell line and immune splenocytes derived from rats immunized either with primary mouse brain cultured cells or membrane components. In cultures one antibody, anti-BSP-2 (Brain Surface Protein-2), was preferentially directed against neurones while another, anti-BSP-3 (Brain Surface Protein-3), preferentially labeled astrocytes. In mouse cerebellar sections, both labeled the surface of Purkinje cells, granule cells and astrocytes. In addition a cytoplasm localization was apparent in granule cells and astrocytes. Another antibody anti-MESA-1 (Mouse Endothelial Surface Antigen-1) reacted exclusively with the surface of endothelial cells lining blood vessels. These data are discussed with reference to the biochemical nature of the corresponding antigens and to known glycoproteins of neural cell membranes.

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References

  1. Eng, L. F., andBigbee, J. W. 1978. Immunohistochemistry of nervous system-specific antigen. Pages 48–98,in Agranoff, B. W., andAprison, M. H. (eds.), Advances in Neurochemistry. Vol. 3, Plenum Press, New York.

    Google Scholar 

  2. Eng, L. F. 1980. The glial fibrillary acidic (GFA) protein,in Bradshaw, R. A., andSchneider, D. M. (eds.), Proteins of the Nervous System, 2nd edition, Raven Press, New York.

    Google Scholar 

  3. Zomzely-Neurath, C. B., andKeller, A. 1977. Nervous system-specific proteins of vertebrates (a search for functions and physiological roles). Neurochem. Res. 2:353–377.

    Google Scholar 

  4. Lagenaur, C., Sommer, I., andSchachner, M. 1980 Subclass of astroglia in mouse cerebellum recognized by monoclonal antibody. Dev. Biol. 79:367–378.

    Google Scholar 

  5. Ghandour, M. S., Labourdette, G., Vincendon, G., andGombos, G. 1981. A biochemical and immunohistological study of S100 protein in developing rat cerebellum. Dev. Neurosci. 4:98–109.

    Google Scholar 

  6. Raff, M. C., Fields, K. L., Hakomori, S. I., Mirsky, R., Pruss R. M., andWinter, J. 1979. Cell-type specific markers for distinguishing and studying neurons and the major classes of glial cells in culture. Brain Res. 174:283–308.

    Google Scholar 

  7. Matus, A., andMughal, S. 1975. Immunohistochemical localization of S100 protein in brain. Nature (Lond.) 258:740–748.

    Google Scholar 

  8. Ghandour, M. S., Langley, O. K., Labourdette, G., Vincendon, G., andGombos, G. 1981 Specific and artefactual cellular localizations of S100 protein: an astrocyte marker in rat cerebellum. Dev. Neurosci. 4:66–78.

    Google Scholar 

  9. Bignami, A., Eng, L. F., Dahl, D., andUyeda, C. T. 1972. Localization of the glial fibrillary acidic protein in astrocytes by immunofluorescence. Brain Res. 43:429–435.

    Google Scholar 

  10. Schmechel, D. E., Brightman, M. W., andMarangos, P. J. (1980) Neurons switch from non-neuronal enolase to neuron specific enolase during differentiation. Brain Res. 190:195–214.

    Google Scholar 

  11. Langley, O. K., andGhandour, M. S. 1981 An immunocytochemical investigation of non-neuronal enolase astrocyte marker. Histochem. J. 13:137–148.

    Google Scholar 

  12. Norenberg, M. D., andMartinez-Hernandez, A. 1979. Fine structural localization of glutamine synthetase in astrocytes of rat brain. Brain Res. 161:303–310.

    Google Scholar 

  13. Ghandour, M. S., Langley, O. K., Vincendon, G., Gombos, G., Filippi, D., Limozin, N., Dalmasso, C., andLaurent, G. 1980. Immunochemical and immunohistochemical study of carbonic anhydrase II in adult rat cerebellum: a marker for oligodendrocytes. Neuroscience 5:559–571.

    Google Scholar 

  14. Langley, O. K., Ghandour, M. S., Vincendon, G., andGombos, C. 1980. Carbonic anhydrase: an ultrastructural study in rat cerebellum. Histochem. J. 12:473–483.

    Google Scholar 

  15. Raff, M. C., Mirsky, R., Fields, K. L., Lisak, R. P., Dorfman, S. H., Silberberg, D. H., Gregson, N. A., Liebowitz, S., andKennedy, M. C. 1978. Galactocerebroside is a specific cell-surface antigenic marker for oligodendrocytes in culture. Nature, 274:813–816.

    Google Scholar 

  16. Leveille, P. J., McGinnis, J. F., Maxwell, D. S., andDevellis, J. 1980 Immunocytochemical localization of glycerol-3-phosphate dehydrogenase in rat oligodendrocytes. Brain Res. 196:287–305.

    Google Scholar 

  17. Sternberger, N. H., Itoyama, Y., Kies, M. W., andDe F. Webster, H. 1978. Myelin Basic protein demonstrated immunocytochemically in oligodendroglia prior to myelin sheath formation. Proc. Natl. Acad. Sci. USA 75:2521–2524.

    Google Scholar 

  18. Roussel, G., Delaunoy, J-P., Mandel, P., andNussbaum, J-L. 1978. Ultrastructural localization study of two Wolfgram proteins in rat brain tissue. J. Neurocytol. 7:155–163.

    Google Scholar 

  19. Gombos, G., andAunis, D. 1981. Enzymes of neurotransmitter metabolism as neuronal markers in the central nervous system. Scand. J. Immunol., Vol. 14, suppl. 9, in press.

  20. Hökfelt, T., Johansson, O., Ljungdahl, Å, Lunberg, J. M., andSchultzberg, M. 1980. Peptidergic neurones Nature Lond. 284:515–521.

    Google Scholar 

  21. Langley, O. K., Ghandour, M. S., Vincendon, G., andGombos, G. 1980. An ultrastructural immunocytochemical study of nerve-specific protein in rat cerebellum. J. Neurocytol. 9:783–798.

    Google Scholar 

  22. Köhler, G. andMilstein, C. 1974. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature (Lond.) 356:495–497.

    Google Scholar 

  23. Barnstable, C. J. 1980. Monoclonal antibodies which recognize different cell types in the rat retina. Nature (Lond.) 286:231–235.

    Google Scholar 

  24. Eisenbarth, G. S., Walsh, F. S., andNirenberg, M. 1979. Monoclonal antibody to a plasma membrane antigen of neurons. Proc. Nat. Acad. Sci. (Wash.) 76:4913–4917.

    Google Scholar 

  25. Kennet, R. H., andGilbert, F. 1979. Hybrid myelomas producing antibodies against a human neuroblastoma antigen present on fetal brain. Science, 203:1120–1121.

    Google Scholar 

  26. Shirefeld, L. A., Sato, U. L., andRosenberg, N. E. 1980. Monoclonal rat antimouse brain antibody detects Abelson murine leukemia virus target cells in mouse bone marrow. Cell 20:11–17.

    Google Scholar 

  27. Vulliamy, T., Rattray, S., andMirsky, R. 1981. Cell surface antigen distinguishes sensory and autonomic peripheral neurones from central neurones. Nature (Lond.) 291:418–420.

    Google Scholar 

  28. Cohen, J., andSelvendran, S. Y. 1981. A neuronal cell-surface antigen is found in the CNS but not in peripheral neurones. Nature 291:421–423.

    Google Scholar 

  29. Lerner, R. A., andBergsma, D. 1978. The molecular basis of cell-cell interaction. A. R. Hiss, New York.

    Google Scholar 

  30. Hirn, M., Pierres, M., Deagostini-Bazin, H., Hirsch, M., andGoridis, C. 1981 Monoclonal antibody against cell surface glycoprotein of neurons. Brain Res. 214:433–439.

    Google Scholar 

  31. Hirn, M., Pierres, M., Deagostini-Bazin, H., Demierre, M., Goridis, C., Ghandour, S., Langley, K., andGombos, G. 1982. A new brain cell surface glycoprotein identified by monoclonal antibody. Neuroscience, 7:239–250.

    Google Scholar 

  32. Ghandour, S., Langley, K., Gombos, G., Hirn, M., Hirsch, M., andGoridis, C. 1982. A surface marker of murine vascular endothelial cells defined by monoclonal antibody. J. Histochem. Cytochem. 30:165–170.

    Google Scholar 

  33. Goridis, C., Hirsch, M., Dosseto, M., andBaechler, E. 1980. Identification and characterization of two surface glycoproteins on cultured cerebellar cells. Brain Res. 182:397–414.

    Google Scholar 

  34. Goridis, C., Soher, M. A., Hirsch, M., andSchachner, M. 1978. Cell surface proteins of cultured brain cells and their recognition by anti-cerebellum antiserum. J. Neurochem. 31:531–539.

    Google Scholar 

  35. Messer, A., andSmith, D. M. 1977. In vitro behaviour of granule cells from Staggerer and Weaver mutants of mice. Brain Res. 130:13–23.

    Google Scholar 

  36. Pierres, M., Kourilsky, F. M., Rebouah, J. P., Dosseto, M., andCaillol, C. 1980. Distinct epitopes on Ik gene products identified by monoclonal antibodies. Europ. J. Immunol. 10:950–957.

    Google Scholar 

  37. Coffino, P., andScharff, M. S. 1971. Rate of somatic mutation in immunoglobulin production by mouse myeloma cells. Proc. Natl. Acad. Sci. USA 68:219–223.

    Google Scholar 

  38. Fu, S. C., Cruz, T. F., andGurd, J. W. 1981. Development of synaptic glycoproteins: effect of postnatal age on the synthesis and concentration of synaptic membrane and synaptic junctional fucosyl and sialyl glycoproteins. J. Neurochem. 36:1338–1351.

    Google Scholar 

  39. Gurd, J. W. 1979. Molecular and biosynthetic heterogeneity of fucosyl glycoproteins associated with rat brain synaptic junctions. Biochim. Biophys. Acta 555:221–229.

    Google Scholar 

  40. Rohrer, H., andSchachner, M. 1980. Surface proteins of cultured mouse cerebellar cells. J. Neurochem. 35:792–803.

    Google Scholar 

  41. Jorgensen, O. S. 1979. Polypeptides of the synaptic membrane antigens D1, D2 and D3. Biochim. Biophys. Acta 181:153–162.

    Google Scholar 

  42. Barclay, N. A., Letarte-Muirhead, M., Williams, A. F., andFaulkes, R. A. 1976. Chemical characterization of the Thy-1 glycoproteins from the membranes of rat thymocytes and brain. Nature (Lond.) 263:563–567.

    Google Scholar 

  43. Ghandour, M. S., Langley, O. K., andVarga, V. 1980. Immunohistological localization of γ-glutamyl transpeptidase in cerebellum at light and electron microscope levels. Neuroscience Lett. 20:125–129.

    Google Scholar 

  44. Orlowski, M., Sessa, G., andGreen, J. P. 1974. γ-glutamyl transpeptidase in brain capillaries: possible site of blood-brain barrier for aminoacids, Science 184:66–68.

    Google Scholar 

  45. Ryan, W. T., Ryan, U. S., Schultz, D. R., Whitaker, C., andChung, A. 1975. Subcellular localization of pulmonary angiotensin-converting enzyme. Biochem. J. 146:497–499.

    Google Scholar 

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Authors and Affiliations

  1. Centre de Neurochimie du CNRS, 5, rue Blaise Pascal, 67000, Strasbourg, France

    O. K. Langley, M. S. Ghandour, G. Gombos, M. Hirn & C. Goridis

  2. Centre d'Immunologie, INSERM-CNRS de Marseille, Luminy Case 906, 13288, Marseille cédex 9, France

    M. Hirn & C. Goridis

Authors
  1. O. K. Langley
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  2. M. S. Ghandour
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  3. G. Gombos
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  4. M. Hirn
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  5. C. Goridis
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Langley, O.K., Ghandour, M.S., Gombos, G. et al. Monoclonal antibodies as neural cell surface markers. Neurochem Res 7, 349–362 (1982). https://doi.org/10.1007/BF00965646

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