Skip to main content
SpringerLink
Log in

Pertussis vaccine reduces agonist binding to the rat heart muscarinic receptor and its guanine nucleotide modulation

  • Original Articles
  • Published:
Neurochemical Research Aims and scope Submit manuscript

Abstract

The injection ofBordetella pertussis, inactivated by merthiolate, causes a 2-fold increase in the IC50 of carbamylcholine (carbachol) in displacing [3H];-L(−) quinuclidinyl benzilate binding ([3H]QNB) to the receptor. In control animals, 50 μM Gpp(NH)p causes a 6-fold decrease in the affinity of carbachol binding, whereas after vaccination the reduction is only 1.6-fold. After pertussis treatment there is no alteration in the affinity and number of [3H]QNB binding sites of to the muscarinic receptor.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Berrie, C. P., Birdsall, N. J. M., Burgen, A. S. V., andHulme, E. C. 1979. Guanine nucleotides regulates receptor binding in the heart. Biochem. Biophys. Res. Commun. 87:1000–10005.

    PubMed  Google Scholar 

  2. De Wildt, D. J., Van der Beek, E. M., de Jong, Y., Nijkamp, F. P., andKreeftemberg, J. G. 1985. Effects of dexamethasone and indomethacin on the vascular beta2-adrenolitic action of pertussis toxin in rats; a prostaglandin-mediated phenomenon. Eur. J. Pharmacol. 108:581–588.

    Google Scholar 

  3. Dunlap, J., andBrown, J. H. 1984. Differences and similarities in muscarinic receptor of rat heart and retina: effects of guanine nucleotides and N-ethylmaleimide, J. Neurochem. 43:214–222.

    PubMed  Google Scholar 

  4. Hazeki, O., andUi, M. 1981. Modification by islet-activating protein of receptor-mediated regulation of cyclic AMP accumulation in isolated rat heart cells. J. Biol. Chem. 256:2856–2862.

    PubMed  Google Scholar 

  5. Hosey, M. M. 1983. Regulation of ligand binding to cardiac muscarinic receptors by ammoniun ion and guanine nucleotides. Biochem. Biophys. Res. Commun. 757:119–127.

    Google Scholar 

  6. Hunier, D. D., andNathanson, N. M. 1984. Decreased physiological sensitivity, mediated newly synthesized muscarinic acetylcholine receptors in embryonic chicken heart. Proc. Natl. Acad. Sci. U.S.A. 81:3582–3586.

    PubMed  Google Scholar 

  7. Kahn, R. A., andGilman, A. G. 1984 ADP-Ribosylation of Gs promotes the dissociation of its alpha and beta subunits. J. Biol. Chem. 259:6235–6240.

    PubMed  Google Scholar 

  8. Katada, T., andUi, M. 1981. Islet-activating protein, a modifier of receptor-mediated regulation of rat islet adenylate cyclase. J. Biol. Chem. 256:8310–8317.

    PubMed  Google Scholar 

  9. Kurose, H., Katada, T., Amano, T. andUi, M. 1983. Specific uncoupling of isletactiviating protein, pertussis toxin, of negative signal translocation via alpha-adrenergic, cholinergic and opiate receptors in neuroblastoma x glioma hybrid cells. J. Biol. Chem. 258:4870–4875.

    PubMed  Google Scholar 

  10. Kurose, H., andUi, M. 1983. Functional uncoupling of muscarinic receptors from adenylate cyclase in rat cardiac membranes by the active component of islet-activating protein, pertussis toxin. 1983. J. Cyclic Nucleotide and Protein Phosphorylation Research. 9:305–318.

    Google Scholar 

  11. Levitzki, A. 1984. Receptor to effector coupling in the receptor-dependent adenylate cyclase system. J. Receptor Res. 4:399–410.

    Google Scholar 

  12. Lowry, O. M., Rosebrough, N. J., Farr, A. L., andRandall, R. J. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265–275.

    PubMed  Google Scholar 

  13. Lujan, M., Lopez, E., Ramirez, R., Aguilar, H., Martinez-Olmedo, M. A., andGarcia-Sainz, J. A. 1984. Pertussis toxin blocks the action of morphine, norepinephrine and clonidine on isolated guinea-pig ileum. 1984. Eur. J. Pharmacol. 100:377–380.

    PubMed  Google Scholar 

  14. Martinez-Olmedo, M. A., andGarcia-Sainz, J. A. 1984. Direct action of pertussis toxin in isolated Hamster fat cells. Eur. J. Pharmacol. 99:115–118.

    PubMed  Google Scholar 

  15. Matsuzawa, M., andNiremberg, M. 1975. Receptor mediated shift in cGMP and cAMP levels in neuroblastoma cells. Proc. Natl. Acad. Sci. USA 72:3472–3476.

    PubMed  Google Scholar 

  16. McMahon, K. K., Green, R. D. andHosey, M. M. 1985. Attenuation of chick heart adenylate cyclase by muscarinic receptor after pertussis toxin treatment. Biochem. Biophys. Res. Commun. 126:622–629.

    PubMed  Google Scholar 

  17. Nathanson, N. M., Klein, W. L., andNiremberg, M. 1978. Regulation of adenylate cyclase activity mediated by acetylcholine receptors. Proc. Natl. Acad. Sci. U.S.A. 75:1788–1791.

    PubMed  Google Scholar 

  18. Olianas, M. C., Onali, P., Neff, N. M., andCosta, E. 1983. Adenylate cyclase activity of synaptic membranes from rat striatum. Inhibition by muscarinic receptor agonists. Mol. Pharmacol. 23:393–398.

    PubMed  Google Scholar 

  19. Olianas, M. C., Onali, P., Neff, N. M., andCosta, E. 1983. Muscarinic receptors modulate dopamine-activated adenylate cyclase of rat striatum. J. Neurochem. 41:1364–1369.

    PubMed  Google Scholar 

  20. Rodbell, M. 1980. The role of hormone receptors and GTP regulatory proteins in membrane transduction. Nature 284:17–22.

    PubMed  Google Scholar 

  21. Rosenberger, L. B., Roeske, W. R., andYamamura, H. I. 1979. The regulation of muscarinic cholinergic receptors by guanine nucleotides in cardiac tissue. Eur. J. Pharmacol. 56:179–180.

    PubMed  Google Scholar 

  22. Tamura, M., Nogimosi, K., Murai, S., Yajima, M., Ito, K., Katada, T., Ui, M., andIshu, S. 1982. Subunit structure of islet-activating protein, pertussis toxin, in conformity with the A-B model. Biochemistry 21:5516–5522.

    PubMed  Google Scholar 

  23. Uchida, S., Matsumoto, K., Mizushima, A., Osugi, T., Higuchi, H., andYoshida, M. 1984. Effects of guanine nucleotide and sulfhydryl reagent on subpopulation of muscarinic acetylcholine receptor in mammalian hearts: possible evidence for interconversion of super-high and low-affinity binding sites. Eur. J. Pharmacol. 100:201–209.

    PubMed  Google Scholar 

  24. Waelbroek, M., Robberech, P., Chatelain, P., andChristophe, J. 1982. Rat cardiac muscarinic receptors. I. Effects of guanine nucleotides on high-and low-affinity binding sites. Mol. Pharmacol. 21:179–180.

    Google Scholar 

  25. Wei, J. W., andSulakhe, P. V. 1979. Agonist-antagonist interactions with rat atrial muscarinic cholinergic receptor sites: differential regulation by guanuine nucleotides. Eur. J. Pharmacol. 58:91–92.

    PubMed  Google Scholar 

  26. Yajima, M., Hosoda, K., Kanbayashi, Y., Nakamura, T., Nogimosi, K., Mizushima, Y., Nakase, Y., andUi, M. 1978. Islets-activating protein (IAP) in Bordetella pertussis that potentiates insulin secretory responses of rats. J. Biochem. 83:295–303.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Instituto de Biologia Celular, Facultad de Medicina (U.B.A.), 1121, Buenos Aires, Argentina

    Jose S. Aguilar, Maria I. Fonseca & Eduardo De Robertis

Authors
  1. Jose S. Aguilar
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Maria I. Fonseca
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Eduardo De Robertis
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Aguilar, J.S., Fonseca, M.I. & De Robertis, E. Pertussis vaccine reduces agonist binding to the rat heart muscarinic receptor and its guanine nucleotide modulation. Neurochem Res 11, 745–752 (1986). https://doi.org/10.1007/BF00965342

Download citation

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00965342

Keywords

Search

Navigation