Abstract
Cord blood mononuclear cells (MNC) were isolated from 20 normal full-term newborns. These MNC were preincubated with either 50, 100, or 200 µg/ml Thymostimulin or without Thymostimulin. The interleukin-2 (IL-2) and γ-interferon (γ-IFN) production, cytotoxicity, and lymphoproliferation and IL-2 receptor (Tac) expression were all significantly increased after Thymostimulin treatment. For evaluation of thein vivo effect, two combined-imimunodeficiency patients defective on the thymic level, one with progressive BCG infection, and one with DiGeorge syndrome were used. Before Thymostimulin treatment, the patient's MNC did not produce sufficient amounts of IL-2 and γ-IFN. The cytotoxicity and lymphoproliferation were also low. After Thymostimulin treatment, the IL-2 and γ-IFN production, cytotoxicity, and lymphoproliferative response were enhanced. These results suggest that Thymostimulin may be beneficial in the clinical treatment of primary cellular immunodeficiency. The improved immune reactivity including cytotoxicity and enhanced IL-2 and γ-IFN production in the Thymostimulin treatment also indicates that there may be a beneficial effect on the combination of chemotherapy and Thymostimulin.
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References
Miller JFAP: Immunological function of the thymus. Lancet 2:748–749, 1961
Bach JF, Dardenne M, Pleau JM, Bach MA: Isolation, biochemical characteristics, and biological activity of a circulating thymic hormone in the mouse and in the human. Ann NY Acad Sci 249:186–192, 1975
Goldstein AL, Slater FD, White A: Preparation, assay, and partial purification of a thymic lymphocytopoietic factor (Thymosin). Proc Natl Acad Sci USA 56:1010–1024, 1966
Goldstein G: Isolation of bovine thymosin: A polypeptide hormone of the thymus. Nature 247:11–14, 1974
Kook AI, Yakir Y, Trainin N: Isolation and partial chemical characterization of THF, a thymus hormone involved in immune maturation of lymphoid cells. Cell Immunol 19:151–156, 1975
Goldstein AL, Guha A, Zatz MM, Hardy MA, White A: Purification and biological activity of thymosin, a hormone of the thymus gland. Proc Natl Acad Sci USA 69:1800–1811, 1972
Stiehm ER, Fulginiti VA: Immunologic Disorders in Infants and Children. Philadelphia, WB Saunders, 1980, p 335
Wara DM, Goldstein AL, Doyle MF, Amman J: Thymosin activity in patients with cellular immunodeficiency. N Engl J Med 292:70–74, 1975
Lin CY, Hsu HC, Hsieh HC: Treatment of progressive Bacillus Calmette-Guérin infection in an immunodeficient infant with a specific bovine thymic extract (Thymostimulin). Pediat Infect Dis 4:402–405, 1985
Lin CY, Hsu CH, Liu KC, Chen CL, Hsu HC: Serial immunologic and histologic studies in the treatment of necrotizing fasciitis with combined immunodefiency by a bovine thymic extract (Thumostimulin). J Pediat Surg 21:1000–1004, 1986
Lin CY, Hsu HC, Chen CL, Shen EY: Treatment of combined immunodeficiency with thymic extract (Thymostimulin). Ann Allergy 58:379–384, 1987
Lin CY, Chu CM: In vitro effect of a bovine thymic extract (Thymostimulin) on T-cell differentiation in cord blood lymphocytes. Pediat Pharmacol 5:181–187, 1985
Bergesi G, Falchetti R: Caratterizzazzione chinica et attivita biologia di un nuovo estratto timico. Folia Allergol Immunol Clin 24:204–209, 1977
Falchetti R, Bergesi A, Eshkol A, Caliero C, Adorini L, Caprino L: Pharmacological and biological properties of a calf thymus extract (TP-1). Drugs Exp Clin Res 3:39–47, 1977
Gillis S, Ferm MM, Ou W, Smith KA: T cell growth factor: Parameters of production and a quantitative microassay for activity. J Immunol 120:2027–2032, 1978
Lin CY: Transient remission after intercurrent measles infection in a patient with hyper-immunoglobulin E syndrome. Pediat Res 20:685–688, 1986
Berger SL, Hitchcock MJM, Zoon KC, Biokemmeier CS, Friedman RM, Chang EM: Characterization of interferon messenger RNA synthesis in Namalva cells. J Biol Chem 255:2955–2961, 1980
Menconi E, Barzi A, Bonmassar E, Pucetti P: Effect of a calf thymic extract (TP-1) on the generation of cell mediated cytotoxicity in vitro.In Thymus, Thymic Hormones and T-Lymphocytes, F Aiuti, H Wigzell, (eds). New York, Academic Press, 1980, p 219
Dardenne M, Pleau JM, Man NK, Bach JF: Structural study of circulating thymic factor: A peptide isolated from pig serum. J Biol Chem 252:8040–8044, 1977
Shoham J: The thymus extract TP-1 and human immune reactivity.In Thymus, Thymic Hormones and T-Lymphocytes, F Aiuti, H Wigzell, (eds). New York, Academi Press, 1980 pp 273–294
Low TLK, Thurman GB, McAdoo M, McClure J, Rossio JL, Naylor PH, Goldstein AL: The chemistry and biology of Thymosin. J Biol Chem 254:981–986, 1979
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Lin, CY., Kuo, YC., Lin, CC. et al. Enhancement of interleukin-2 and γ-interferon productionin vitro on cord blood lymphocytes andin vivo on primary cellular immunodeficiency patients with thymic extract (Thymostimulin). J Clin Immunol 8, 103–107 (1988). https://doi.org/10.1007/BF00917897
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DOI: https://doi.org/10.1007/BF00917897