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A membrane vesicle/ribosome preparation fromSerratia marcescens elicits peritoneal exudate cells expressing both tumoricidal and bactericidal activity

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Abstract

A biological response modifier called ImuVert, derived from the bacteriumSerratia marcescens, produced long-lasting elevation of peritoneal excludate cell (PEC) numbers after intraperitoneal injection into mice. These cells had enhanced ability to phagocytose both latex beads and opsonizedListeria monocytogenes. PEC harvested 2–14 days after a single injection of ImuVert killedL. monocytogenes, and ImuVert protected mice from infection byL. monocytogenes, measured both by LD50 and bacterial growth in vivo. Cells harvested 7 and 14 days after ImuVert injection also were tumoricidal, measured as killing of P815 mastocytoma cells, and ImuVert induced macrophages to express tumoricidal properties in vitro. These data suggest that ImuVert has a unique ability to induce a chronic inflammatory response, as other agents do not induce such a long-lasting influx of bactericidal inflammatory cells that also show tumoricidal activity. The consequences of this response appear to include protection from infection by certain bacteria.

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References

  1. Mackaness, G. B. 1969. The influence of immunologically committed lymphoid cells on macrophage activity in vivo.J. Exp. Med. 129:973–992.

    Google Scholar 

  2. Newborg, M. F., andR. J. North. 1980. On the mechanism of T-cell independent antiListeria resistance in nude mice.J. Immunol. 124:571–576.

    Google Scholar 

  3. Czuprynski, C. J., P. M. Henson, andP. A. Campbell. 1985. Enhanced accumulation of inflammatory neutrophils and macrophages mediated by transfer of T cells from mice immunized withListeria monocytogenes.J. Immunol. 134:3449–3544.

    Google Scholar 

  4. Stevenson, M. M., P. A. L. Kongshavn, andE. Skamene. 1981. Genetic linkage of resistanceto Listeria monocytogenes with macrophage inflammatory responses.J. Immunol. 127:402–407.

    Google Scholar 

  5. Campbell, P. A., C. J. Czuprynski, andJ. L. Cook. 1984. Differential expression of macrophages effector functions: Bactericidal versus tumoricidal activities.J. Leukocyte Biol. 36:293–306.

    Google Scholar 

  6. Campbell, P. A., B. P. Canono, andJ. L. Cook. 1988. Mouse macrophages stimulated by recombinant gamma interferon to kill tumor cells are not bactericidal for the facultative intracellular bacteriumListeria monocytogenes.Infect. Immun. 56:1371–1375.

    Google Scholar 

  7. Havell, E. A. 1989. Evidence that tumor necrosis factor has an important role in antibacterial resistance.J. Immunol. 143:2894–2899.

    Google Scholar 

  8. Wing, E. J., I. D. Gardner, F. W. Ryning, andJ. S. Remington. 1977. Dissociation of effector functions in populations of activated macrophages.Nature 268:479–485.

    Google Scholar 

  9. Hopper, K. E., andJ. M. Cahill. 1983. Immunoregulation by macrophages. II. Separation of mouse peritoneal macrophages having tumoricidal and bactericidal properties and those secreting PGE and interleukin-1.J. Reticuloendothelial. Soc. 33:443–456.

    Google Scholar 

  10. Czuprynski, C. J., P. M. Henson, andP. A. Campbell. 1984. Killingof Listeria monocytogenes by inflammatory neutrophils and mononuclear phagocytes from immune and non-immune mice.J. Leukocyte Biol. 35:193–208.

    Google Scholar 

  11. Alford, C. E., T. K. King, Jr., andP. A. Campbell. 1991. Role of transferrin, transferrin receptors, and iron in macrophage listericidal activity.J. Exp. Med. 174:459–466.

    Google Scholar 

  12. Campbell, P. A. 1986. Are inflammatory phagocytes responsible for resistance to facultative intracellular bacteria?Immunol. Today 7:70–72.

    Google Scholar 

  13. Tilney, L. G., andD. A. Portnoy. 1989. Actin filaments and the growth, movement, and spread of the intracellular bacterial parasite,Listeria monocytogenes. J. Cell. Biol. 109:1597–1608.

    Google Scholar 

  14. Adams, D. O., andT. A. Hamilton. 1984. The cell biology of macrophage activation.Annu. Rev. Immunol. 2:283–318.

    Google Scholar 

  15. Hibbs, J. B., Jr., R. R. Taintor, H. A. Chapman, Jr., andJ. B. Weinberg. 1977. Macrophage tumor killing: Influence of the local environment.Science 197:270–282.

    Google Scholar 

  16. Ruco, L. P., andM. S. Meltzer. 1977. Macrophage activation for tumor cytotoxicity: Induction of tumoricidal macrophages by PPD in BCG immune mice.Cell. Immunol. 32:203–215.

    Google Scholar 

  17. Warren, R. P., C. A. McCall., andR. W. Urban. 1989. Augmentation of natural killer activity by ImuVert: A biological response modifier derived fromSerrada marcescens. Mol. Biolher. 1:145–151.

    Google Scholar 

  18. Warren, R. P., C. A. McCall, andR. W. Urban. 1989. Augmentation of ADCC and cytotoxic T-cell activity with ImuVert.Mol. Biolher. 1:323–327.

    Google Scholar 

  19. Hirschfield, G. R., D. W.Smiley, S. A.Baldwin, R. C.Lawson, M. S.Hindahl, and F. C.Pearson. Structural and immunological properties of theSerraria marcescens derived biological response modifier ImuVert (submitted).

  20. Reed, L. J., andH. Meunch. 1938. A simple method of estimating 50 percent endpoints.Am. J. Hyg. 27:493.

    Google Scholar 

  21. Riches, D. W. H., andP. M. Henson. 1986. Bacterial lipopolysaccharide suppresses the production of catalytically active lysosomal acid hydrolases in human macrophages.J. Cell Biol. 102:1606–1614.

    Google Scholar 

  22. Riches, D. W. H., P. H. Henson, L. K. Remigio, J. F. Catterall, andR. C. Strunk. 1988. Differential regulation of gene expression during macrophage activation with a polyribonucleotide.J. Immunol. 141:180–188.

    Google Scholar 

  23. Baker, L. A., andP. A. Campbell. 1980. Thioglycollate medium decreases resistance to bacterial infection in mice.Infect. Immun. 27:455–460.

    Google Scholar 

  24. DRevets, D. A., andP. A. Campbell. 1991. Roles of complement and complement receptor type 3 in phagocytosis ofListeria monocytogenes by inflammatory mouse peritoneal macrophages.Infect. Immun. 59:2645–2652.

    Google Scholar 

  25. Nowotny, A. T., P. H. Keler, E. Pham, A. Kovats, O. Aiello, H. Shonekan, H. FriedMan, andA. G. Johnson. 1988. Isolation of a non-endotoxic antitumor preparation fromSerrana marcescens. J. Biol. Response Modifiers 7:296–308.

    Google Scholar 

  26. D'Hinterland, L. D., G.Normier, and A. M.Pinel. Method of preparing immunostimulant proteoglycans which induce production of interferon, proteoglycans obtained and pharmaceutical compositions containing them. U.S. Patent No. 4,501,693.

  27. Mccall, C. A., L. Weimer, andS. Baldwin. 1989. Stimulation of cytokine production by the bacterially derived biological response modifier ImuVert.Cytokine 1:113.

    Google Scholar 

  28. House, R. V., F. C.Pearson, and P. T.Thomas. 1990. Selective potentiation of host resistance in mice following treatment with ImuVert.Mol. Biolher. (in press).

  29. Czuprynski, C. J., J. F. Brown, K. M. Young, A. J. Cooley, andR. S. Kurtz. 1988. Effects of murine recombinant interleukin 1-alpha on the host response to bacterial infection.J. Immunol. 140:962–968.

    Google Scholar 

  30. Haak-Frendscho, M., K. M. Young, andC. J. Czuprynski. 1989. Treatment of mice with human recombinant interleukin-2 augments resistance to the facultative intracellular pathogenListeria monocytogenes.Infect. Immun. 57:3014–3021.

    Google Scholar 

  31. Nakano, Y., K. Onozuka, Y. Terada, H. Shinomiya, andM. Nakano. 1990. Interactions between endogenous gamma interferon and tumor necrosis factor in host resistance against primary and secondaryListeria monocytogenes infections.Infect. Immun. 57:3331–3337.

    Google Scholar 

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Authors and Affiliations

  1. Cell Technology Inc., 80301, Boulder, Colorado

    Catherine McCall, Lori Weimer & Susan Baldwin

  2. The National Jewish Center for Immunology and Respiratory Medicine, 1400 Jackson St., 80206, Denver, Colorado

    David W. H. Riches, Beth Canono & Priscilla A. Campbell

Authors
  1. Catherine McCall
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  2. Lori Weimer
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  3. Susan Baldwin
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  4. David W. H. Riches
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  5. Beth Canono
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  6. Priscilla A. Campbell
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Supported in part by USPHS grant AI-11240 (PAC).

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McCall, C., Weimer, L., Baldwin, S. et al. A membrane vesicle/ribosome preparation fromSerratia marcescens elicits peritoneal exudate cells expressing both tumoricidal and bactericidal activity. Inflammation 16, 355–369 (1992). https://doi.org/10.1007/BF00917627

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