Abstract
The effects of fluoride (F) on neutrophil protuberance formation and induced Con A acceptor molecule migration were assessed microscopically. Below 5 mM, F had little effect on acceptor migration, while it markedly inhibited formation of colchicine-induced protuberances. The anion also increased the rate at which preformed protuberances regressed. Since protuberance formation is enhanced by disassembly of microtubules, these data suggest that F promotes and/or stabilizes microtubule assembly. Microtubule assembly is favored by binding of GTP to tubulin subunits, while GDP binding favors disassembly of microtubules. Since F binds with GDP, forming a new complex that mimics GTP, the anion would be expected to enhance microtubule assembly. Over the same F concentration range, the anion failed to inhibit acceptor polarization, but did inhibit cytochalasin B-enhanced dispersion of prepolarized Con A acceptors, implying that, at low concentrations, F also affected microfilament cycling. Concentrations of F in excess of 5 mM inhibited acceptor migration as well as protuberance formation. At 20 mM, the anion abolished both events, yet at this same concentration F induced neutrophil superoxide generation and degranulation, suggesting that acceptor migration is not a prerequisite for these two neutrophil effector activities.
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References
Bretscher, M. S. 1984. Endocytosis: Relation to capping and cell locomotion.Science 224:681–686.
Loor, F. L. 1974. Binding and redistribution of lectins on lymphocyte membranes.Eur. J. Immunol 4:210–220.
Oliver, J. M., R. Lalchandani, andE. L. Becker. 1977. Actin redistribution during concanavalin A cap formation in rabbit neutrophils.J. Reticuloendothel. Soc. 21:359–364.
Schreiner, G. F., andE. R. Linanue. 1976. Membrane and cytoplasmic changes in B lymphocytes induced by ligand-surface immunoglobulin interaction.Adv. Immunol. 24:37–155.
Walter, R. J., R. D. Berlin, andJ. M. Oliver. 1980. Asymmetric Fc receptor distribution on human PMN oriented in a chernotactic gradient.Nature 286:724–725.
Weinbaum, D. L., J. A. Sullivan, andG. L. Mandell. 1980. Receptors for concanavalin A cluster at the front of polarized neutrophils.Nature 286:725–727.
Hoffstein, S. T. 1981. The role of microtubules and microfilaments in lysosomal enzyme release from polymorphonuclear leukocytes.Methods Cell Biol. 23:259–282.
Goldstein, I., S. T. Hoffstein, J. Gallin, andG. Weissmann. 1973. Mechanism of lysosomal enzyme release from human leukocytes: microtubule assembly and membrane fusion induced by a component of complement.Proc. Natl. Acad. Sci. U.S.A. 70:2916–2920.
Howard, T. H., andW. Meyer. 1984. Chemotactic peptide modulation of actin assembly and locomotion in neutrophils.J. Cell Biol. 98:1265–1271.
Gabler, W. L., H. R. Creamer, andW. W. Bullock. 1986. Modulation of the kinetics of induced neutrophil superoxide generation by fluoride.J. Dent. Res. 65:1159–1165.
Gabler, W. L., H. R.Creamer, and W. W.Bullock. 1983. Fluoride modulation of neutrophil degranulation.Reticuloendothel. Soc. Abstract. 37.
Wilkinson, P. C. 1983. Effects of fluoride on locomotion of human blood leukocytes in vitro.Arch. Oral Biol. 28:415–418.
Curnutte, B. D., B. M. Babion, andM. L. Karnorsky. 1979. Fluoride-mediated activation of the respiratory burst in neutrophils. A reversible process.J. Clin. Invest. 63:637–647.
Gabler, W. L., H. R.Creamer, and W. W.Bullock. 1988. Fluoride activation of neutrophils, similarities to formyl-methionyl-leucyl-phenylalanine.Inflammation (in press).
Bullock, W. W., W. L. Gabler, andH. R. Creamer. 1982. A rapid quantiative assay for activated neutrophils.Inflammation 6:305–318.
DePetris, S. 1974. Inhibition and reversal of capping by cytochalasin B, vinblastine and colchicine.Nature 250:54–56.
Hays, T. S., andE. D. Salman. 1986. The stabilization of microtubules in isolated spindles by tubulin-colchicine complex.Cell Motil. Cytoskel. 6:282–290.
Margolis, R. L., andL. Wilson, 1981. Regulation of microtubule steady state in vitro by ATP,Cell 18:673–679.
Zurier, R. B., S. Hoffstein, andG. Weissmann. 1973. Mechanisms of lysosomal enzyme release from human leukocytes. I. Effects of cyclic nucleotides and colchicine.J. Cell Biol. 58:27–41.
Spilberg, I., B. Mandell, andS. Hoffstein. 1979. A proposed model for chemotactic deactivation. Evidence for microtubule modulation of polymorphonuclear leukocyte chemotaxis.J. Lab. Clin. Med. 94:361–369.
Kirschnen, M., andT. Mitchison. 1986. Beyond self-assembly: From microtubules to morphogenesis.Cell 45:329–342.
Allende, J. E. 1988. GTP-mediated macromolecular interactions: The common features of different systems.FASEB J. 2:2356–2367.
Snyderman, R., C. D. Smith, andM. W. Verghese. 1987. Model for leukocyte regulation by chemoattractant receptors: Roles of a quanine nucleotide regulatory protein and phosphoinositide metabolism.J. Leuko. Biol. 40:785–800.
Howlett, A. C., P. C. Sternweiss, B. A. Macik, P. M. Van Arsdale, andA. G. Gilman. 1979. Reconstruction of catecholamine-sensitive adenylate cyclase.J. Biol. Chem. 254:2287–2295.
Downes, R. W., A. M. Spiegel, M. Singker, S. Reen, andG. D. Aurback. 1980. Fluoride stimulation of adenylate cyclase is dependent on the quanine nucleotide regulatory protein.J. Biol. Chem. 255:949–954.
DePetris, S. 1975. Concanavalin A receptors, immunoglobulins and O-antigen of the lymphocyte surface. Interactions with concanavalin A and cytoplasmic structures.J. Cell Biol. 65:123–146.
Gabler, W. L., andHunter, N. 1987. Inhibition of human neutrophil phagocytosis and intracellular killing of yeast cells by fluoride.Arch. Oral Biol. 32:363–366.
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This research was supported by a grant from the Medical Research Foundation of Oregon
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Gabler, W.L., Mugrditchian, M., Creamer, H.R. et al. Effect of fluoride on movement of concanavalin A-acceptor molecules of human neutrophils. Inflammation 13, 317–328 (1989). https://doi.org/10.1007/BF00914398
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DOI: https://doi.org/10.1007/BF00914398