Summary
Previously, we reported that cardiac troponin T (TnT) can be detected and measured in coronary effluent from isolated rat hearts during hypoxia. The present study was designed to evaluate the release kinetics of TnT from post-ischemic rat hearts. Using the Langendorff technique, the hearts were reperfused for 4h after 20 min or 60 min of global ischemia. Coronary flow was measured by timing the collection of the coronary perfusate that dripped from the hearts, and left ventricular pressure (LVP) was monitored continuously during the experiments. The amount of TnT released in 1 min was compared with the release of creatine kinase (CK) and lactate dehydrogenase (LD). The release kinetics of CK and LD showed a monophasic pattern and the levels at 4 h after reperfusion returned to baseline levels. By contrast, the release kinetics of TnT showed a small peak followed by a larger and more sustained peak. There were good negative correlations between developed pressure of LVP and both Σ TnT and the amount of TnT released within 1 min at 4 h after reperfusion. These results indicate that the release kinetics of TnT is different from that of CK and LD during reperfusion, and further that cardiac TnT is a useful indicator of myocardial cell damage and can be used to evaluate the degree of myocardial cell damage in both the early and late phase of acute myocardial infarction.
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References
Katus HA, Remppis A, Looser S, Hallermeier K, Scheffold T, Kubler W (1989) Enzyme linked immunoassay of cardiac troponin T for detection of acute myocardial infarction in patients. J Mol Cell Cardiol 21:1349–1353
Katus HA, Remppis A, Scheffold T, Diederich KW, Kuebler W (1991) Intracellular compartmentation of cardiac troponin T and its release kinetics in patients with reperfused and non-reperfused myocardial infarction. Am J Cardiol 67:1360–1367
Mair J, Artner-Dworzak E, Lechleitner P, Smidt J, Wagner I, Dienstl F, Puschendorf B (1991) Cardiac troponin T in diagnosis of acute myocardial infarction. Clin Chem 37:845–852
Asayama J, Yamahara Y, Ohta B, Miyazaki H, Tatsumi T, Matsumoto T, Inoue D, Nakagawa M (1992) Release kinetics of cardiac troponin T in coronary effluent from isolated rat hearts during hypoxia and reoxygenation. Basic Res Cardiol 87:428–436
Asayama J, Tatsumi T, Yamahara Y, Matsumoto T, Miyazaki H, Sakai R, Inoue M, Omori I, Inoue D, Nakagawa M (1992) Effects of reduction of contractile work on mechanical function in post-hypoxic guinea pig papillary muscles and on myocardial energy metabolism in post-ischemic rat hearts. Jpn Circ J 56:292–300
Katus HA, Looser S, Hallermayer K, Remppis A, Borgya A, Essig U, Geub U (1992) Development and in vitro characterization of a new immunoassay of cardiac troponin T. Clin Chem 38: 386–393
Rosalki SB (1967) An improved procedure for serum creatine phosphokinase. J Lab Clin Med 69:696–700
Wroblewski F, LaDue JS (1955) Lactic dehydrogenase in blood. Proc Soc Exp Biol Med 90:210–213
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Yamahara, Y., Asayama, J., Ohta, B. et al. Release kinetics and correlation with hemodynamic dysfunction of cardiac troponin T in coronary effluent from isolated rat hearts during reperfusion. Basic Res Cardiol 88, 307–313 (1993). https://doi.org/10.1007/BF00800637
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DOI: https://doi.org/10.1007/BF00800637