Abstract
An automated system, TRAKCELL, was developed for the quantitation of cells in culture. It enabled cell counting, classification according to morphological cell characteristics and measurement of cell proliferation and differentiation. The system was tested on the toxic effect of ascorbic acid on rat brain catecholaminergic neurons in primary culture. In parallel, the effects of nerve growth factor, dexamethasone and forskolin on cell differentiation were studied using rat pheochromocytoma PC12 cells. The results show that the system permits rapid and reproducible measurements of cell density and of the morphological changes observed following various drug treatments.
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Abbreviations
- DEX:
-
dexamethasone
- FSK:
-
forskolin
- NGF:
-
nerve growth factor
- TH:
-
tyrosine hydroxylase
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Xu-van Opstal, W.Y., Billardon, C., Caillaud, T. et al. Automatic cell culture quantitation with TRAKCELL: application to cell toxicology and differentiation. Cell Biol Toxicol 10, 387–392 (1994). https://doi.org/10.1007/BF00755787
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DOI: https://doi.org/10.1007/BF00755787