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Studies on the histochemical “leucine aminopeptidase” reaction

III. On the different LNA-splitting enzymes from spleen

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Zeitschrift für Zellforschung und mikroskopische Anatomie Abt. Histochemie Aims and scope Submit manuscript

Summary

Fractionation experiments on fresh beef spleen homogenates have been performed with the aim to separate the LNA-splitting group of enzymes from the regular aminopeptidases and cathepsin C. The results indicate that the LNA-splitting enzymes are distinct from the LA-splitting aminopeptidases, and further that the group of LNA-splitting enzymes is heterogeneous with respect to the effect of added cysteine + EDTA.

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References

  • Fruton, J. S., andM. J. Mycek: Studies on beef spleen cathepsin C. Arch. Biochem.65, 11–20 (1956).

    Google Scholar 

  • Gutmann, H. R., andJ. S. Fruton: On the proteolytic enzymes of animal tissues. VIII. An intracellular enzyme related to chymotrypsin. J. biol. Chem.174, 851–858 (1948).

    Google Scholar 

  • Haba, G. de la, P. S. Cammarata andS. N. Timasheff: The partial purification and some physical properties of cathepsin C from beef spleen. J. biol. Chem.234, 316–319 (1959).

    Google Scholar 

  • Patterson, E. K., S.-H. Hsiao andA. Keppel: Studies on dipeptidases and aminopeptidases. I. Distinction between leucine aminopeptidase and enzymes which hydrolyse L-leucyl-β-naphthylamide. J. biol. Chem.238, 3611–3620 (1963).

    Google Scholar 

  • Sylvén, B., andI. Bois: Studies on the histochemical “Leucine Aminopeptidase” reaction. II. Chemical and histochemical comparison of the enzymatic and environmental factors involved. Histochemie3, 341–353 (1963).

    Google Scholar 

  • ——, andI. Bois: Studies on the histochemical “Leucine Aminopeptidase” reaction. I. Identity of the enzymes possibly involved. Histochemie3, 65–78 (1962).

    Google Scholar 

  • ——, andO. Snellman: A new metal-dependent enzyme present in cathepsin C preparations. Biochim. biophys. Acta (Amst.)65, 350–353 (1962).

    Google Scholar 

  • Tallan, H. H., M. E. Jones andJ. S. Fruton: On the proteolytic enzymes of animal tissues. X. Beef spleen cathepsin C. J. biol. Chem.194, 792–805 (1952).

    Google Scholar 

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Authors and Affiliations

  1. The Cancer Research Division of Radiumhemmet, Karolinska Institute, Stockholm 60, Sweden

    B. Sylvén & O. Snellman

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  1. B. Sylvén
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  2. O. Snellman
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Sylvén, B., Snellman, O. Studies on the histochemical “leucine aminopeptidase” reaction. Histochemie 3, 484–486 (1964). https://doi.org/10.1007/BF00736629

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  • DOI: https://doi.org/10.1007/BF00736629

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