Skip to main content
SpringerLink
Log in

Determination of anti-AGE antibodies in human serum

  • Glycosylation And Disease Papers
  • Published:
Glycoconjugate Journal Aims and scope Submit manuscript

Abstract

The aim of this study was to develop an immunoenzyme method for the determination of anti-AGE antibodies in human serum. Human aortic elastin glycatedin vitro (AGE-elastin) was used as an antigen, expressing AGE-epitopes, common to all glycated proteins.

Polyclonal serum from guinea-pig against AGE-Hemocyanin was obtained according to Nakayamaet al. [(1989)Biochem Biophys Res Commun 162: 740–45] and its specificity was tested via direct and competitive ELISA. Sera of 20 type 1 diabetic patients and 20 healthy subjects were tested using the method described. Seventeen patients had elevated levels of competing factors that may be anti-AGE antibodies, compared with the healthy group. The method could be used for investigation of different clinical groups of type 1 diabetic patients. Such a study would help in understanding the pathogenic role of autoantibodies against advanced glycation end products of proteins for the development of long-term diabetic complications.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Vlassara H, Brownlee M, Cerami A (1986)Clin Chem 32: B37-B41.

    Google Scholar 

  2. Vishwanath V, Frank K, Elmets C, Dauchot P, Monnier V (1986)Diabetes 35: 916–21.

    Google Scholar 

  3. Eble A, Thorpe S, Baynes J (1983)J Biol Chem 258: 9406–12.

    Google Scholar 

  4. Schnider S, Kohn R (1982)Exp Gerontol 17: 185–94.

    Google Scholar 

  5. Brownlee M, Cerami A, Vlassara H (1988)N Engl J Med 318: 1315–21.

    Google Scholar 

  6. Brownlee M, Pongor S, Cerami A (1983)J Exp Med 158: 1739–44.

    Google Scholar 

  7. Brownlee M, Vlassara H, Cerami A (1985)Diabetes 34: 938–41.

    Google Scholar 

  8. Bucala R, Tracey K, Cerami A (1991)J Clin Invest 87: 432–38.

    Google Scholar 

  9. Yang Z, Makita Z, Horii Y, Brunelle S, Cerami A, Sehajpal P, Suthanthiran M, Vlassara H (1991)J Exp Med 174: 515–24.

    Google Scholar 

  10. Horii Y, Skolnik E, Suthanthiran M, Vlassara H (1992)Diabetes 41: 59A.

  11. Peak P, Charlsworth J, Timmermans V, Deckert T (1989)Diabetes Res Clin Pract 11: 109–14.

    Google Scholar 

  12. Kirstein M, Van Deventer S, Vlassara H (1990)J Cell Biol 89: 14E.

  13. Vlassara H, Bucala R, Liliane S (1994)Lab Invest 70: 138–51.

    Google Scholar 

  14. Skolnik E, Yang Z, Makita Z, Radoff S, Kirstein M, Vlassara H (1991)J Exp Med 174: 931–39.

    Google Scholar 

  15. Nakayama H, Taneda S, Kunajima S, Aoki S, Kuroda I, Misawa K, Nakagawa S (1989)Biochem Biophys Res Commun 162: 740–45.

    Google Scholar 

  16. Starcher B, Galione M (1976)Anal Biochem 74: 441–47.

    Google Scholar 

  17. Partridge S, Davis H, Adair G (1955)Biochem J 61: 11.

    Google Scholar 

  18. Baydanoff S, Konova E, Dosheva I, Dorovski P (1994) Glycosylation & Dis1: 53–58.

    Google Scholar 

  19. Baydanoff S, Konova E, Benchev I, Atanasova M, Tzakov I (1994) NATO Advanced Study Institute, Vascular Endothelium, June 18–27, Crete, Greece.

Download references

Author information

Authors and Affiliations

  1. Department of Biology/Immunology, University School of Medicine, Pleven, Bulgaria

    Steffan Baydanoff & Emiliana Konova

  2. Department of Neurology, University School of Medicine, Pleven, Bulgaria

    Natalia Ivanova

Authors
  1. Steffan Baydanoff
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Emiliana Konova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Natalia Ivanova
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Baydanoff, S., Konova, E. & Ivanova, N. Determination of anti-AGE antibodies in human serum. Glycoconjugate J 13, 335–339 (1996). https://doi.org/10.1007/BF00731464

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00731464

Keywords

Search

Navigation