Abstract
The study of the changes in UV absorbance of DNA solutions in water/dioxane and water/ethylene glycol mixture at different concentrations shows that the thermal denaturation of DNA is sensitive to the electrical permittivity of the media and the water content. At relative low concentrations of co-solvent the dominant feature is the electrical permittivity. When water content is lower than a critical value, the electrical permittivity is no longer the determinant of the denaturation temperature but the partial volume fraction of water. The critical water content is about 0.69 partial volume fraction of water.
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de Xammar Oro, J.R., Grigera, J.R. On the thermal stability of DNA in solution of mixed solvents. J Biol Phys 21, 151–154 (1995). https://doi.org/10.1007/BF00712343
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DOI: https://doi.org/10.1007/BF00712343