Summary
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1.
Autoradiographic techniques coupled with computerized microdensitometry and comparison with125I standards were used to characterize and quantitate receptors for neuropeptides in rat brain and adrenal and pituitary glands.
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2.
These techniques are rapidly performed, anatomically precise, and more sensitive than membrane binding techniques. They permit the determination of complete saturation curves and Scatchard analysis in discrete nuclei of the rat brain and in single rat pituitary and adrenal glands.
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3.
Angiotensin II (AII) receptors were quantitated after incubation of 16-µm tissue sections with the AII agonist125I-[Sar1]-AII.
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4.
High-affinity, high-density AII receptors were present in the organon subfornicalis, organon vasculosum laminae terminalis and nuclei triangularis septalis, suprachiasmatis, and paraventricularis of the rat and in rat adrenal capsule-zona glomerulosa area, adrenal medulla, and anterior pituitary.
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5.
These techniques could be used for precise localization and quantitation of other neuropeptide receptors in single rat brain nuclei, after optimizing the assay conditions and provided that suitable125I ligands are available.
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References
De Souza, E. B., Perrin, M. H., Insel, T. R., Rivier, J., Vale, W. W., and Kuhar, M. J. (1984). Corticotropinreleasing factor receptors in rat forebrain: Autoradiographic identification.Science 2241449–1451.
Dufy-Barbe, L., Rodriguez, F., Arsaut, J., Verrier, D., and Vincent, J. D. (1982). Angiotensin stimulates prolactin release in the rhesus monkey.Neuroendocrinology 35242–247.
Fitzsimons, J. T. (1980). Angiotensin stimulation of the central nervous system.Rev. Physiol. Biochem. Pharmacol. 87117–167.
Goochee, C., Rasband, W., and Sokoloff, L. (1980). Computerized densitometry and color coding of [14C]deoxyglucose autoradiograms.Ann. Neurol. 7359–370.
Hauger, R. L., Aguilera, G., Baukal, A. J., and Catt, K. J. (1982). Characterization of angiotensin II receptors in the anterior pituitary gland.Mol. Cell. Endocrinol. 25203–212.
Helke, C. J., Shults, C. W., Chase, T. N., and O'Donohue, T. L. (1984). Autoradiographic localization of substance P receptors in rat medulla: Effect of vagotomy and nodose ganglionectomy.Neuroscience 12215–223.
Israel, A., Correa, F. M. A., Niwa, M., and Saavedra, J. M. (1984a). Quantitative determination of angiotensin II binding sites in rat brain and pituitary gland by autoradiography.Brain Res. 322341–345.
Israel, A., Correa, F. M. A., Niwa, M., and Saavedra, J. M. (1984b). Quantitative measurement of angiotensin II (AII) receptors in discrete regions of rat brain, pituitary and adrenal gland by autoradiography.Clin. Exp. Hyper.- Theory Pract. A6(10, 11):1761–1764.
Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. (1951). Protein measurement with the Folin phenol reagent.J. Biol. Chem. 193265–275.
Mendelsohn, F. A. O., Aguilera, G., Saavedra, J. M., Quirion, R., and Catt, K. J. (1983). Characteristics and regulation of angiotensin II receptors in pituitary, circumventricular organs and kidney.Clin. Exp. Hyper. Theory Pract. A51081–1097.
Mendelsohn, F. A. O., Quirion, R., Saavedra, J. M., Aguilera, G., and Catt, K. J. (1984). Autoradiographic localization of angiotensin II receptors in rat brain.Proc. Natl. Acad. Sci. USA 811575–1579.
Mukherjee, A., Kulkarni, P., McCann, S. M., and Negro-Vilar, A. (1982). Evidence for the presence and characterization of angiotensin II receptors in rat anterior pituitary membranes.Endocrinology 110665–667.
Munson, P. J. (1983). Ligand: A computerized analysis of ligand binding data. InMethods in Enzymology, Vol. 92. Immunochemical Techniques. Part E (J. J. Langone and H. Van Vunakis, Eds.), Academic Press, New York, pp. 543–576.
Palacios, J. M., Niehoff, D. L., and Kuhar, M. J. (1981). Receptor autoradiography with tritium-sensitive film: Potential for computerized densitometry.Neurosci. Lett. 25101–105.
Phillips, M. I. (1978). Angiotensin in the brain.Neuroendocrinology 25354–377.
Rainbow, T. C., Bleisch, W. V., Biegon, A., and McEwen, B. S. (1982). Quantitative densitometry of neurotransmitter receptors.J. Neurosci. Methods 5127–138.
Rainbow, T. C., Parsons, B., and Wolfe, B. B. (1984). Quantitative autoradiography ofβ 1- andβ 2-adrenergic receptors in rat brain.Proc. Natl. Acad. Sci. USA 811585–1589.
Scott Young, W., III, and Kuhar, M. J. (1979). A new method for receptor autoradiography: [3H]Opioid receptors in rat brain.Brain Res. 179255–270.
Sirett, N. E., McLean, A. S., Bray, J. J., and Hubbard, J. I. (1977). Distribution of angiotensin II receptors in rat brain.Brain Res. 122299–312.
Steele, M. K., Negro-Vilar, A., and McCann, S. M. (1981). Effect of angiotensin II on in vivo and in vitro release of anterior pituitary hormones in the female rat.Endocrinology 109893–899.
Tran, V. T., Uhl, G. R., Perry, D. C., Manning, D. C., Vale, W. W., Perrin, M. H., Rivier, J. E., Martin, J. B., and Snyder, S. H. (1984). Autoradiographic localization of somatostatin receptors in rat brain.Eur. J. Pharmacol. 101307–309.
Unnerstall, J. R., Niehoff, D. L., Kuhar, M. J., and Palacios, J. M. (1982). Quantitative receptor autoradiography using [3H]Ultrofilm: Application to multiple benzodiazepine receptors.J. Neurosci. Methods 659–73.
Zarbin, M. A., Innis, R. B., Wamsley, J. K., Snyder, S. H., and Kuhar, M. J. (1983). Autoradiographic localization of cholecystokinin receptors in rodent brain.J. Neurosci. 3877–906.
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Israel, A., Plunkett, L.M. & Saavedra, J.M. Quantitative autoradiographic characterization of receptors for angiotensin II and other neuropeptides in individual brain nuclei and peripheral tissues from single rats. Cell Mol Neurobiol 5, 211–222 (1985). https://doi.org/10.1007/BF00711007
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DOI: https://doi.org/10.1007/BF00711007