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The role ofSaccharomyces cerevisiae Cdc40p in DNA replication and mitotic spindle formation and/or maintenance

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Abstract

Successful progression through the cell cycle requires the coupling of mitotic spindle formation to DNA replication. In this report we present evidence suggesting that, inSaccharomyces cerevisiae, theCDC40 gene product is required to regulate both DNA replication and mitotic spindle formation. The deduced amino acid sequence ofCDC40 (455 amino acids) contains four copies of a β-transducin-like repeat. Cdc40p is essential only at elevated temperatures, as a complete deletion or a truncated protein (deletion of the C-terminal 217 amino acids in thecdc40-1 allele) results in normal vegetative growth at 23°C, and cell cycle arrest at 36°C. In the mitotic cell cycle Cdc40p is apparently required for at least two steps: (1) for entry into S phase (neither DNA synthesis, nor mitotic spindle formation occurs at 36°C and (2) for completion of S-phase (cdc40::LEU2 cells cannot complete the cell cycle when returned to the permissive temperature in the presence of hydroxyurea). The role of Cdc40p as a regulatory protein linking DNA synthesis, spindle assembly/maintenance, and maturation promoting factor (MPF) activity is discussed.

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Authors and Affiliations

  1. Dept. of Biology, Technion, 32000, Haifa, Israel

    Nora Vaisman, Andrey Tsouladze, Kenneth Robzyk & Yona Kassir

  2. Molecular Microbiology and Biotechnology, Tel-Aviv University, 69978, Ramat-Aviv, Israel

    Sigal Ben-Yehuda & Martin Kupiec

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  1. Nora Vaisman
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  2. Andrey Tsouladze
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  3. Kenneth Robzyk
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  4. Sigal Ben-Yehuda
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  5. Martin Kupiec
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  6. Yona Kassir
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Communicated by C. Hollenberg

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Vaisman, N., Tsouladze, A., Robzyk, K. et al. The role ofSaccharomyces cerevisiae Cdc40p in DNA replication and mitotic spindle formation and/or maintenance. Molec. Gen. Genet. 247, 123–136 (1995). https://doi.org/10.1007/BF00705642

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  • DOI: https://doi.org/10.1007/BF00705642

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