Abstract
The results of an experimental study of nonlinear dynamic processes in the electron transfer system, the reaction centers (RCs) of purple bacteria are presented. A difference was observed in the absorption spectra of RCs exposed to a rising intensity of acting light compared to a descending intensity of acting light. We observed the hysteresis of the RC optical transmission coefficient at λ=865 nm, with a quasistationary increase and subsequent decrease of the optical excitation level. The kinetics of charge recombination in an RC containing two quinone acceptors revealed a dependence on the prehistory of the RC illumination. The results were interpreted in terms of the existence of a light-induced memory effect in the electron-conformational system and the appearance of bifurcation in the system at critical values of the photoinduced electron flux through the macromolecule.
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References
Chernavskaya, N.M. and Chernavskii, D.S.,Tunnel Electron Transport in Photosynthesis, Moscow University Press, Moscow, 1977.
McElroy, J.D., Mauzerall, D.C. and Feher, G., Characterization of primary reactions in bacterial photosynthesis. II. Kinetic Studies of light-induced EPR signal (g 210026) and the optical absorbance changes at cryogenic temperatures,Biochim. Biophys. Acta 333 (1974), 261–277.
Noks, P.P., Lukashev, E.P., Kononenko, A.A., Venediktov, P.S. and Rubin, A.B., On the possible role of macromolecular components in functioning of the photosynthetic reaction centers of purple bacteria,Molekulyarnaya biologiya 11 (1977), 1090–1099.
Kleinfeld, D., Okamura, M.Y. and Feher, G., Electron-transfer kinetics in photosynthetic reaction centers cooled to cryogenic temperatures in the charge-separated state: Evidence for light-induced changes,Biochemistry 23 (1984), 5780–5786.
Shaitan, K.V., Uporov, K.V., Lukashev, E.P., Kononenko, A.A. and Rubin, A.B., Photoconformational transition — the reason of thermal and light effects in charge recombination processes in reaction centers of photosynthetic bacteria,Molekulyarnaya biologiya 25 (1991), 695–705.
Gaididei, Yu.B. and Kharkyanen, V.N.: Nonlinear effects of the electron transport in biomolecular system, Preprint ITP-88-130p, Kiev, 1988.
Goushcha, A.O., Kapustina, M.T. and Kharkyanen, V.N., Nonlinear effect of dynamic self-organization in macromolecular systems caused by photocontrolled electron flux,J. Biol. Phys. 19 (1994), 273–283 (this issue).
Petrov, E.G., Kharkyanen, V.N., Noks, P.P., Kononenko, A.A., Venediktov, P.S. and Rubin, A.B., Kinetic model of electron and conformational transitions in the reaction centers of purple bacteria,Izvest. AN SSSR, ser. biolog., No. 1 (1983), 28–43.
Lukashev, E.P., Timopheev, K.N., Kononenko, A.A., Venediktov, P.S. and Rubin, A.B., Temperature dependence of the reduction kinetics of photooxidized reaction centre bacteriochlorophyll in dark and light adapted chromatophores of purple bacteria,Photosynthetica 10 (1976), 423–430.
Zakharova, N.I., Fabian, M.Ya., Uspenskaya, N.Ya., Kononenko, A.A. and Rubin, A.B., Structural and functional characteristics of photosynthetic reaction centers separated with lauryldimethylamidoxin fromRps. Sphaeroides (wild type),Biokhimiya 46 (1981), 1703–1711.
Clayton, R.K., Primary processes in bacterial photosynthesis,Ann. Rev. Biophys. Bioenerg. 2 (1973), 131–156.
Parson, W.W.,Biochim. Biophys. Acta 153 (1967), 248–259.
Morrison, L.E. and Loach, P.A.,Photochem. Photobiol. 27 (1978), 751–757.
Okamura, M.Y. and Feher, G., Proton transfer in reaction centers from photosynthetic bacteria,Ann. Rev. Biochem. 61 (1992), 861–896.
Wraight, C.A. and Stein, R.R.,FEBS Lett. 113 (1980), 73–77.
Okamura, M.Y., Debus, R.I., Kleinfeld, D. and Feher, G., inFunction of Quinones in Energy Conserving Systems, Academic Press, New York, 1982, pp. 299–317.
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Dobrovolskii, A.A., Filippov, A.G., Goushcha, A.O. et al. Experimental evidence of dynamic self-organization in the electron transfer system (example of reaction centers of purple bacteria). J Biol Phys 19, 285–293 (1993). https://doi.org/10.1007/BF00700667
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DOI: https://doi.org/10.1007/BF00700667