Abstract
Although Oparin used coacervate droplets from two or more types of polymer to model the first cell, he hypothesized homacervation from protein, consistent with Pasteur and Darwin. Herrera made two amino acids and numerous cell-like structures (“sulfobes”) in the laboratory, which probably arose from intermediate polymers. Our experiments have conformed with a homoacervation of thermal proteinoid, in which amino acid sequences are determined by the reacting amino acids themselves. All proteinoids that have been tested assemble themselves alone in water to protocells. The protocells have characteristics of life defined by Webster's Dictionary: metabolism, growth, reproduction and response to stimuli in the environment. The protocells are able also to evolve to more modern cells including the initiation of a nucleic acid coding system.
Principal spinoffs from the results are revised evolutionary theory, models for protoneurons and networks thereof, and numerous industrial applications of thermal polyamino acids. Life itself has thus been reaffirmed to be rooted in protein, not in DNA nor RNA, which are however crucial to inheritance in modern life as “instruction manual” (Kornberg).
Recognition of the advances have been considerably delayed by the deeply held assumption that life began by chance from random polymerization of amino acids, in contrast to the experimental findings. The concepts of DNA/RNA-first and protein-first are reconciled by a rise-and-fall progression as often seen in biochemical and biological evolution.
The fact that amino acids order themselves explains in turn that thermal copolyamino acids are finding numerous applications. The entire sequence of processes in the proteinoid origins theory is now seen to be highly deterministic, in close accord with Einstein.
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References
Bergmann, M. and Fraenkel-Conrat, H. (1937) The role of specificity in the enzymic synthesis of proteins—synthesis with intracellular enzymesJ. Biol. Chem. 119, 707–720.
Berra, T.M. (1990)Evolution and the Myth of Creationism, Stanford University Press, Stanford.
Bonner, W. (1991) The origin and amplification of biomolecular chirality.Origins Life Evol Biosphere 21, 59–111.
Canning, P. and Marks, F.M. (eds.) (1992)Who's Who in America. pp. 1129.
Crick, F. (1981)Life Itself Its Origin and Nature. Simon and Schuster, New York.
De Duve, C. (1991)Blueprint for a Cell/The Nature and Origin of Life. Neil Patterson Publishers, Burlington NC.
De Fontaine, D. and Fox, S.W. (1954). Application of a quantitative method of peptide analysis to the N-terminal sequence of lysozyme.J. Amer. Chem. Soc. 76, 3701–3703.
Dose, K. and Rauchfuss, H. (1972) On the electrophoretic behavior of thermal polymers of amino acids, in D.L. Rohlfing and A.I. Oparin (eds.)Molecular Evolution/Prebiological and Biological, pp. 199–217.
Dose, K. and Zaki, L. (1971) Peroxidative and catalatic activity of proteinoids.Z. Naturforsch 26B, 144–147.
Dyson, F. (1985)Origins of Life. Cambridge University Press.
Edman, P. (1950) Method for determination of the amino acid sequence in peptides.Acta Chem. Scand. 4, 283–293.
Eigen, M. (1971) Selforganization of matter and the evolution of biological macromolecules.Naturwissenschaften 58, 149–212.
Eigen, M. (1986) The physics of molecular evolution, in H. Baltscheffsky, H. Jornvall, and R. Rigler (eds.)Molecular Evolution of Life, Cambridge University Press, pp. 13–41.
Eirich, F. R. (1994) Some thoughts on the origin of life on Earth.This Conference.
Ferris, J.P. (1993) Catalysis and prebiotic RNA synthesis.Origins of Life / Evolution of Biosphere 23, 307–315.
Fox, S.W. (1945) Terminal amino acids in peptides and proteins.Advances in Protein Chemistry 2, 155–177.
Fox, S. W (1953) A correlation of observations suggesting a familial mode of molecular evolution as a concomitant of biological evolution.Amer. Naturalist 87, 253–256.
Fox, S. W. (1957) The chemical problem of spontaneous generation.J. Chem. Education 34, 472–479.
Fox, S. W. (1959) Biological overtones of the thermal theory of biochemical origins.Bull. Amer. Inst. Biol.Sc.9, 20–23.
Fox, S. W. (1960) How did life begin?Science 132, 200–208.
Fox, S. W. (1971) Chemical origins of cells-2.Chem. Eng. News 49, 46–53.
Fox, S. W. (1974) Aleksandr Ivanovich Oparin,Encyclopedia Britannica, 15th edition, pp. 577–578.
Fox, S. W. (1981) A model for protocellular coordination of nucleic acid and protein syntheses. In M. Kageyama, K. Nakamura, T. Oshima, and T. Uchida (eds.)Science and Scientists. Japan Sc. Soc. Press, Tokyo, pp. 39–45.
Fox, S. W. (1988)The Emergence of Life/Darwinian Evolution From the Inside. Basic Books.
Fox, S. W. (1992) Thermal proteins in the first life and in the “mind-body” problem. In K. Haefner (ed.)Evolution of Information Processing Systems. Springer Verlag, pp. 203–228.
Fox, S. W. (1994) To cellular life and neurocellular assemblies in B. Poglazov (ed.)Biochemistry of the 21st Century: Problems and Frontiers. Nova Press.
Fox, S. W. (1994a) Thermal synthesis of amino acids and the origin of life.Geochim. Cosmochim. Acta, submitted.
Fox, S.W. and Bahn, P.R. (1994) Thermal polyamino acids in the origin of life and biomedical applications. In E. Bittar and E. Bittar (eds.)Principles of Medical Biology. Jai Press.
Fox, S.W. and Dose, K. (1977)Molecular Evolution and the Origin of Life, revised edition, Marcel Dekker, New York.
Fox, S.W. and Harada, K. (1960) The thermal copolymerization of amino acids common to protein.J. Amer. Chem. Soc., 3745–3751.
Fox, S.W. and Middlebrook, M. (1954) Anhydrocopolymerization of amino acids under the influence of hypothetically primitive terrestrial conditions.Federation Proc. 13, 703.
Fox, S.W. and Pappelis, A. (1993). Synthetic molecular evolution and protocells.Quart. Rev. Biol. 68, 79–82.
Fox, S.W. and Wax, H. (1950) Enzymic synthesis of peptide bonds. III. The relative effects of some amino acids and their acyl substituents.J. Amer. Chem. Soc. 72, 5087–5090.
Fox, S.W., Winitz, M., and Pettinga, C.W. (1953) Enzymic synthesis of peptide bonds. VI. The influence of residue type on papain-catalyzed reactions of some benzoylamino acids with some amino acid anilides.J. Amer. Chem. Soc. 75, 5539–5542.
Fox, S.W., Johnson, J.A. and Vegotsky, A. (1956) On biochemical origins and optical activity.Science 124, 923–925.
Fox, S.W., Vegotsky, A., Harada, K., and Hoagland. P.D. (1957) Spontaneous generation of anabolic pathways, protein, and nucleic acid.Ann. N.Y. Acad. Sci. 69, 328–337.
Fox, S.W., Harada, K., and Kendrick, J. (1959) Production of spherules from synthetic proteinoid and hot water.Science 129, 1221–1223.
Fox, S.W., McCauley, R.J., Montgomery, P.O'B., Fukushima, T., Harada, K., and Windsor, C.R. (1969) Membrane-like properties in microsystems assembled from synthetic protein-like polymer. In F. Snell, J. Wolken, G.J. Iverson, and J. Lam (eds.)Physical Principles of Biological Membranes. Gordon and Breach, pp. 417–432.
Fox, S.W., Harada, K., and Hare, P.E. (1972) Amino acid precursors in lunar fines from Apollo 14 and earlier missions.Geochim. Cosmochim, Acta (Suppl. 3)2, 2109–2118.
Francis, S., Margulis, L., and Barghoorn, E.S. (1978) On the experimental silicification of microorganisms. II. On the time of appearance of eukaryotic organisms in the fossil record.Precambrian Res. 6, 65–100.
Gove, P.B. (1966)Webster's Third New International Dictionary, G. and C Merriam Co., pp. 1306.
Heinz, B., Ried, W., and Dose, K. (1980) Thermal production of pteridines and flavins from amino acid mixtures.Angew. Chem. 91, 510–511.
Herrera, A.L. (1942) A new theory of the origin of life.Science 96, 14.
Ingram. V.L. (1957) Chemical difference between normal human and sickle cell anemia hemoglobins.Natl. Acad. Sci. Natl Research Council Publication no.557, 232–252.
Ishima, Y., Przybylski, A.T. and Fox, S.W. (1981) Electrical membrane phenomena in spherules from proteinoid and lecithin.BioSystems 13, 243–251.
Ivanov, O.C. and Förtsch. B. (1986) Universal regularities in protein primary structure.Origins of Life 17, 35–49.
Ivanov, O. and Ivanov, C. (1991) On the most ancient stages of protein evolution.Dokl. Bulg. Akad. Nauk 44, 49–51 (Engl).
Joyce, G.F., Schwartz, A.W., Miller, S.L., and Orgel, L.E. The case for an ancestral genetic system involving simple analogs of the nucleotides.Proc. Natl. Acad. Sci. USA 84, 4398–4402.
Jungck, J.R. and Fox, S.W. (1973) Synthesis of oligonucleotides by proteinoid microspheres acting on ATP.Naturwissenschaften 60, 4256–427.
Kornberg, A. (1989)For the Love of Enzymes Harvard University Press.
Kritsky, M. (1994) Contribution of coenzyme molecules to the evolution of photoreceptors.This conference.
Langenbeck, W. (1935)Die Organischen Katalyzatoren. Springer, Berlin, p. 97.
Lehninger, A.L. (1975)Biochemistry, Worth and Co., New York.
Miller, S. and Orgel, L. (1974)The Origins of Life on the Earth, Prentice-Hall.
Nakashima, T. and Fox. S.W. (1980) Synthesis of peptides from amino acids and ATP with lysine-rich proteinoid.J. Molec. Evolution 15, 161–168.
Negron-Mendoza, A. (1994) Alfonso L. Herrera, a Mexican pioneer in the studies of the origin of life.This conference.
Oparin, A.I. (1957)The Origin of Life on the Earth. Academic Press.
Oparin, A.I. (1966)The Origin and Early Development of Life (transl), Meditsina, Moscow.
Orgel, L. E. (1986) RNA catalysis and the origin of life.J. Theoret. Biol. 123, 127–149.
Pappelis, A. (1994) Domain protolife: protocells and metaprotocells within thermal protein matrices.This conference, program. p. 5.
Rohlfing, D.L. (1975) Coacervate-like microspheres from lysine-rich proteinoid.Origins of Life 6, 203–209
Rohlfing, D.L. (1976) Thermal polyamino acids: synthesis at less than 100° C.Science 193, 68–70.
Sagan, C., Thompson, W.R., and Khare, B.N. (1992) Titan: a laboratory for prebiological organic ehmistry.Accounts Chem. Research 25: 286–292.
Schwartz, A.W. and Fox, S.W. (1964) Thermal synthesis of internucleotide phosphodiester linkages.Biochim. Biophys. Acta87, 694–696.
Sikes, C.S. (1994) Symposium onThermal Polyamino Acids, The Emergence of Life, and Industrial Applications. American Chemical Society Meeting, San Diego.
Sikes, C.S. and Wheeler, A.P. (1991)Surface Reactive Peptides and Polymers/Discovery and Commercialization. ACS Books, Washington.
Steinbach, H.B. (1965) Introductory remarks. In Fox, S.W. (ed.)The Origins of Prebiological Systems and of Their Molecular Matrices. Academic Press, pp. 329–330.
Tyagi, S. and Ponnamperuma, C. (1990) Nonrandomness in prebiotic peptide synthesis.J. Molec. Evolution 30, 391–399.
Vaughan, G., Przybylski, A.T., and Fox, S.W. (1987) Thermal proteinoids as excitability-inducing materials.BioSystems 20, 219–223.
Yuasa, S. and Oro, J. (1981) HCN as a possible precursor of the amino acids in lunar samples. In M. Kageyama, K. Nakamura, T. Oshima, and T. Uchida (eds.)Science and Scientists Japan Sc. Soc. Press, Tokyo, pp. 31–37.
Zubay, G. (1993)Biochemistry, third edition, Wm. C.Brown, Publ.
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Fox, S.W., Bahn, P.R., Dose, K. et al. Experimental retracement of the origins of a protocell. J Biol Phys 20, 17–36 (1995). https://doi.org/10.1007/BF00700418
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DOI: https://doi.org/10.1007/BF00700418