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Recombinational hotspot specific to female meiosis in the mouse major histocompatibility complex

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Abstract

Thewm7 haplotype of the major histocompatibility complex (MHC), derived from the Japanese wild mouseMus musculus molossinus, enhances recombination specific to female meiosis in theK/A β interval of the MHC. We have mapped crossover points of fifteen independent recombinants from genetic crosses of thewm7 and laboratory haplotypes. Most of them were confined to a short segment of approximately 1 kilobase (kb) of DNA between theA β3 andA β2 genes, indicating the presence of a female-specific recombinational hotspot. Its location overlaps with a sex-independent hotspot previously identified in theMus musculus castaneus CAS3 haplotype. We have cloned and sequenced DNA fragments surrounding the hotspot from thewm7 haplotype and the corresponding regions from the hotspot-negative B10.A and C57BL/10 strains. There is no significant difference between the sequences of these three strains, or between these and the published sequences of the CAS3 and C57BL/6 strains. However, a comparison of this Aβ3/Aβ2 hotspot with a previously characterized hotspot in theEβ gene revealed that they have a very similar molecular organization. Each hotspot consists of two elements, the consensus sequence of the mouse middle repetitive MT family and the tetrameric repeated sequences, which are separated by 1 kb of DNA.

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Author notes

  1. Naoto Hanzawa

    Present address: Institute for Marine Biotechnology, Hongoh, Bunkyou-ku, 113, Tokyo, Japan

Authors and Affiliations

  1. Department of Cell Genetics, National Institute of Genetics, Yata-1111, 411, Mishima, Japan

    Toshihiko Shiroishi, Naoto Hanzawa, Tomoko Sagai & Kazuo Moriwaki

  2. Division of Cell Fusion, Department of Cell Biology, National Institute for Basic Biology, Nishigonaka 38, 444, Myodaiji-cho, Okazaki, Japan

    Masahiro Ishiura

  3. Department of Evolutionary Genetics, National Institute of Genetics, Yata-1111, 411, Mishima, Japan

    Takashi Gojobori

  4. Central Research Units, F. Hoffmann-La Roche & Co. Ltd., CH-4002, Basel, Switzerland

    Michael Steinmetz

Authors
  1. Toshihiko Shiroishi
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  2. Naoto Hanzawa
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  3. Tomoko Sagai
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  4. Masahiro Ishiura
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  5. Takashi Gojobori
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  6. Michael Steinmetz
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  7. Kazuo Moriwaki
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Additional information

The nucleotide sequence data reported in this paper have been submitted to the DNA Data Bank of Japan nucleotide sequence database and have been assigned the accession numbers d90007-9.

Offprint requests to: T. Shiroishi.

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Shiroishi, T., Hanzawa, N., Sagai, T. et al. Recombinational hotspot specific to female meiosis in the mouse major histocompatibility complex. Immunogenetics 31, 79–88 (1990). https://doi.org/10.1007/BF00661217

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  • DOI: https://doi.org/10.1007/BF00661217

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