Abstract
The role of systemin inPin2 gene expression was analyzed in wild-type plants of potato (Solanum tuberosum L.) and tomato (Lycopersicon esculentum Mill.), as well as in abscisic acid (ABA)-deficient tomato (sitiens) and potato (droopy) plants. The results showed that systemin initiates Pin2 mRNA accumulation only in wildtype tomato and potato plants. As in the situation after mechanical wounding,Pin2 gene expression in ABA-deficient plants was not activated by systemin. Increased endogenous levels of jasmonic acid (JA) and accumulation of Pin2 mRNA were observed following treatment with α-linolenic acid, the precursor of JA biosynthesis, suggesting that these ABA mutants still have the capability to synthesize de novo JA. Measurement of endogenous levels of ABA and JA showed that systemin leads to an increase of both phytohormones (ABA and JA) only in wild-type but not in ABA-deficient plants.
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Abbreviations
- ABA:
-
(±)cis,trans-abscisic acid
- JA:
-
jasmonic acid
- Pin2:
-
proteinase inhibitor II
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We are specially indebted to Dr. C.A. Ryan (Institute of Biological Chemistry, Washington State University, Pullman, USA) for generously supplying us with the peptide systemin and for helpful comments on the manuscript. We are grateful to Dr. R. Trethewey and N. Provart (Institut für Genbiologische Forschung, Berlin, Germany) for critically reading the manuscript. We also wish to thank Regina Breitfeld for taking care of the greenhouse plants and Antja Voigt for the photographic work.
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Peña-Cortés, H., Prat, S., Atzorn, R. et al. Abscisic acid-deficient plants do not accumulate proteinase inhibitor II following systemin treatment. Planta 198, 447–451 (1996). https://doi.org/10.1007/BF00620062
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DOI: https://doi.org/10.1007/BF00620062