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Synthesis of oligonucleotides containing 3′-terminal nucleotides with inverted configurations of the sugar residues

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Chemistry of Natural Compounds Aims and scope

Abstract

To study the substrate specificity of T4 DNA ligase a series of short DNA duplexes each of which contains in the ligation site a nucleoside with a modified sugar residue is proposed. The synthesis has been performed on previously undescribed mixed dinucleoside phosphates containing deoxycytidine and one of the following modified nucleosides: deoxyxylothymidine and xylo-, lyxo-, and arabinouridines. By the phosphotriester method in solution, using the above-mentioned dinucleoside phosphates, nonanucleotides containing at the 3′-end nucleosides with inverted configurations at the carbon atoms in positions 2′ and 3′ have been obtained.

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Authors
  1. A. V. Tsytovich
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  2. T. S. Oretskaya
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  3. N. G. Dolinnaya
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  4. N. I. Sokolova
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  5. Z. A. Shabarova
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Additional information

M. V. Lomonosov Moscow State University. Translated from Khimiya Priroldnykh Soedinenii, No. 3, pp. 421–425, 1987.

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Tsytovich, A.V., Oretskaya, T.S., Dolinnaya, N.G. et al. Synthesis of oligonucleotides containing 3′-terminal nucleotides with inverted configurations of the sugar residues. Chem Nat Compd 23, 349–352 (1987). https://doi.org/10.1007/BF00600838

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  • DOI: https://doi.org/10.1007/BF00600838

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