Summary
The complete nucleotide sequence (1731 nucleotides) of the gene encoding colicin E7 (cea) of plasmid CoIE7-K317 was determined. This sequence encoded a deduced polypeptide of 576 amino acids of molecular weight 61349 Da. Comparison of the nucleotide and amino acid sequences ofcea E7 with those of other E-group colicins revealed that colicin E7 was closely related to colicin E2, both in gene sequence and in predicted secondary structure of the deduced protein. Judging from the results of cross-immunity tests, we postulated that CoIE7 is probably a proximate ancestor of Co1E2 and Co1E8. Based on results from colicin production tests on cells harboring a 5′ end deleted form of thecea E7 gene, we propose, that a previously unknown, non-inducible promoter may be involved in regulation of the constitutive expression of thecea E7 gene.
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References
Akutsu A, Masaki H, Ohta T (1989) Molecular structure and immunity specificity of colicin E6, an evolutionary intermediate between E-group colicins and cloacin DF13. J Bacteriol 171:6430–6436
Birnboim HC (1983) A rapid alkaline extraction procedure for screening plasmid DNA. Methods Enzymol 100B:243–255
Bolivar F, Backman K (1979) Plasmids ofEscherichia coli as cloning vectors. Methods Enzymol 68:245–267
Chak KF, James R (1984) Localization and characterization of a gene on the CoIE3-CA38 plasmid that confers immunity to colicin E8. J Gen Microbiol 130:701–710
Chak KF, James R (1986) Characterization of the CoIE9-J plasmid and analysis of its genetic organization. J Gen Microbiol 132:61–71
Chak KF, Kuo WS, Lu FM, James R (1991) Characterization of CoIE7 plasmid. J Gen Microbiol 137:91–100
Chan PT, Lebowitz J (1983) The coupled use of “footprinting” and exonuclease III methodology for RNA polymerase binding and initiating. Application for the analysis of three tandem promoters at the control region of colicin E1. Nucleic Acids Res 11:1099–1116
Chen EY, Seeburg PH (1985) Supercoil sequencing: fast and simple method for sequencing plasmid DNA. DNA 4:165–170
Cole ST, Saint-Joanis B, Pugsley AP (1985) Molecular characterization of the colicin E2 operon and identification of its products. Mol Gen Genet 198:465–472
Elzen PJM van den, Walters HHB, Veltkamp E, Nijkamp JJ (1983) Molecular structure and function of the bacteriocin gene and bacteriocin protein of plasmid C1oDF13. Nucleic Acids Res 11:2465–2477
Fredericq P (1949) Sur la resistance croisee entre colicine E et bacteriophage II. C R Soc Biol (Paris) 143:1011–1013
Garoff H, Frischauf AM, Simons K, Lehrach H, Delius H (1980) The capsid protein of Semliki Forest virus has clusters of basic amino acids and prolines in its amino-terminal region. Proc Natl Acad Sci USA 77:6376–6380
Gascuel O, Golmard JL (1988) A simple method for predicting the secondary structure of globular proteins: implications and accuracy. Comp Appl Biosci 4:357–365
Henikoff S (1984) Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28:351–359
Higgins DG, Sharp PM (1988) CLUSTAL: a package for performing multiple sequence alignment on a microcomputer. Gene 73:237–244
Horback E, Muller-Hill B (1988) Insertion of d(pCpG)n · d(pCpG)n into thelac Z gene ofEscherichia coli inhibits expression of β-galactosidasein-vivo. J Mol Biol 202:157–160
Horwitz MSE (1989) Transcription regulationin-vitro by anE. coli promoter containing a DNA cruciform in the “−35” region. Nucleic Acids Res 17:5537–5545
Horwitz MSE, Loeb LA (1988) AnE. coli promoter that regulates transcription by DNA superhelix-induced cruciform extrusion. Science 241:703–705
Innis MA, Myambo KB, Gelfand DDH, Brow MAD (1985) DNA sequencing withThermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA. Proc Natl Acad Sci USA 85:9436–9440
James R, Schneider J, Cooper PC (1987) Characterization of three group A klebicin plasmids: localization of their E colicin immunity genes. J Gen Microbiol 133:2253–2262
Kennedy CK (1971) Induction of colicin production by high temperature or inhibition of protein synthesis. J Bacteriol 108:10–19
Konisky J (1982) Colicins and other bacteriocins with established modes of action. Annu Rev Microbiol 36:125–144
Little JW, Mount DW (1982) The SOS regulatory system ofEscherichia coli. Cell 29:11–22
Masaki H, Ohta T (1985) Colicin E3 and its immunity genes. J Mol Biol 182:217–227
Masaki H, Toba M, Ohta T (1985) Structure and expression of the ColE2-P9 immunity gene. Nucleic Acids Res 13:1623–1635
Messing J (1979) Recombinant DNA Tech Bull 2:43–46
Myers EW, Miller W (1988) Optimal alignment in linear space. Comp Appl Biosci 4:11–17
Ohno-Iwashita Y, Imahori K (1980) Assignment of the functional loci in colicin E2 and E3 molecules by the characterization of its proteolytic fragments. Biochemistry 19:652–659
Ohno-Iwashita Y, Imahori K (1982) Assignment of the functional loci in the colicin El molecule by characterization of its proteolytic fragments. J Biol Chem 257:6446–6451
Peck LJ, Wang JC (1985) Transcriptional block caused by a negative supercoiling induced structural change in an alternating CG sequence. Cell 40:129–137
Pugsley AP, Oudega B (1987) Methods for studying colicins and their plasmids. In: Hardy KG (ed) Plasmids — A practical approach. IRL Press, Oxford, pp 105–161
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467
Shine J, Dalgarno L (1975) Determinant of cistron specificity in bacterial ribosomes. Nature 254:34–38
Steitz JA, Jakes K (1975) How ribosomes select initiator regions in mRNA: Base-pair formation between the 3′ terminus of 168 rRNA and the mRNA during initiation of protein synthesis inEseherichia coli. Proc Natl Acad Sci USA 72:4734–4738
Tabor S, Richardson CC (1987) DNA sequence analysis with a modified bacteriophage T7 DNA polymerase. Proc Natl Acad Sci USA 84:4767–4771
Toba M, Masaki H, Ohta T (1988) Colicin E8, a DNase which indicates an evolutionary relationship between colicins E2 and E3. J Bacteriol 170:3237–3242
van Rompuy L, Min Jou W, Huylebroeck D, Devos R, Fiers W (1981) Complete nucleotide sequence of the nucleoprotein gene from the human influenza strain A/PR/8/34 (HON1). Eur J Biochem 116:347–353
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Soong, BW., Lu, FM. & Chak, KF. Characterization of thecea gene of the CoIE7 plasmid. Molec. Gen. Genet. 233, 177–183 (1992). https://doi.org/10.1007/BF00587577
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DOI: https://doi.org/10.1007/BF00587577