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Three types of sympathetic preganglionic neurones with different electrophysiological properties are identified by intracellular recordings in the cat

  • Excitable Tissues and Central Nervous Physiology
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Abstract

Intracellular recordings were obtained from sympathetic preganglionic neurones (SPN) of the third thoracic segment in cats. Based on differences in their active and passive electrophysiological properties, three different types of SPNs were discerned:

  1. 1)

    Type A neurones had a high resting membrane potential (RMP) (−60 to −86 mV) and a low input resistance (RN) 12–23 MΩ). Action potentials of these neurones had a pronounced IS-SD inflexion and a prominent shoulder in their falling phase. Spikes were rarely generated from the on-going synaptic activity.

  2. 2)

    Type B neurones had a lower RMP (−48 to −65 mV) and a higher RN (21–37 MΩ). Their action potentials were characterized by an after-depolarization; they showed a slight IS-SD inflexion and a less pronounced shoulder in their falling phase. The after-depolarization was abolished by membrane hyperpolarization in a time dependent way. A hyperpolarization of at least 50 ms duration was required for its abolition. The after-depolarization was also abolished during repetitive discharges. In most of these neurones spikes were generated at irregular intervals and low rates (0.06–4.6 spikes/s) from the synaptic activity.

  3. 3)

    Type C neurones were similar to type B neurones, but their action potentials did not show the after-depolarization. Additionally, spikes were generated at fairly regular intervals and rather high rates (0.8–6.5 spikes/s).

The rate of spike repolarization of all neurones was markedly increased by hyperpolarization and decreased by membrane depolarization. Current-voltage curves of some type B and C neurones showed a marked rectification upon membrane hyperpolarization. In all type B and C neurones the presence of the A-current is indicated by the voltage trajectory at the end of hyperpolarizing current pulses. Current-frequency curves of type B and C neurones revealed discharge rates of SPNs as high as 100 spikes/s.

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Authors and Affiliations

  1. I. Physiologisches Institut, Universität Heidelberg, Im Neuenheimer Feld 326, D-6900, Heidelberg, Federal Republic of Germany

    K. Dembowsky, J. Czachurski & H. Seller

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  1. K. Dembowsky
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  2. J. Czachurski
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  3. H. Seller
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Dembowsky, K., Czachurski, J. & Seller, H. Three types of sympathetic preganglionic neurones with different electrophysiological properties are identified by intracellular recordings in the cat. Pflugers Arch. 406, 112–120 (1986). https://doi.org/10.1007/BF00586671

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  • DOI: https://doi.org/10.1007/BF00586671

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