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The distribution of potassium, sodium and chloride across the apical membrane of renal tubular cells: effect of acute metabolic alkalosis

  • Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands
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Abstract

Studies were undertaken to define the effect of acute metabolic alkalosis (hypertonic sodium bicarbonate i.v.) on the chemical gradients for potassium, sodium and chloride across the apical membrane of individual renal tubule cells. Electron microprobe analysis was used on freeze-dried cryosections of the rat renal cortex to measure electrolyte concentrations in proximal tubule cells and in the various cell types of the superficial distal tubule. Analyses were also performed in fluid samples obtained by micropuncture from proximal and early and late distal collection sites. Compared with the appropriate controls (hypertonic sodium chloride i.v.), administration of sodium bicarbonate resulted only in small and mostly insignificant increases in cell potassium concentrations and induced only minor alterations in the cell/tubule fluid potassium concentration gradient for all cell types analysed. This observation suggests that under this condition factors other than an increase in cell potassium concentration are important in modulating potassium transfer across the apical membrane of potassium secreting cells. Nevertheless, since in alkalosis phosphorus and cell dry weight were decreased, and hence cell volume increased, in all but the intercalated cells, actually the potassium content of most tubular cells was higher under this condition. In comparison with animals infused with isotonic saline at low rates (hydropenic controls), infusion of either hypertonic sodium chloride or sodium bicarbonate led to a sharp increase in distal tubule fluid sodium concentrations and in the sodium concentrations of distal convoluted tubule, connecting tubule and principal cells, indicating that under both conditions the primary event causing enhanced transepithelial sodium absorption is stimulation of the sodium entry step. The ensuing rise in cell sodium concentration shold lead secondarily to stimulation of active basolateral sodium extrusion. Intercalated cell sodium concentration was higher only in alkalosis which supports the notion that this cell type is not involved in transepithelial sodium transport.

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Authors and Affiliations

  1. Physiologisches Institut der Universität München, Pettenkoferstrasse 12, D-8000, München 2, Germany

    Franz-X. Beck, Adolf Dörge, Marion Schramm & Klaus Thurau

  2. Medical School, Department of Physiology and Biophysics, University of Alabama in Birmingham, University Station, 35294, Birmingham, AL, USA

    Roger Rick

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  1. Franz-X. Beck
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  2. Adolf Dörge
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  3. Roger Rick
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  4. Marion Schramm
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  5. Klaus Thurau
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Beck, FX., Dörge, A., Rick, R. et al. The distribution of potassium, sodium and chloride across the apical membrane of renal tubular cells: effect of acute metabolic alkalosis. Pflugers Arch. 411, 259–267 (1988). https://doi.org/10.1007/BF00585112

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  • DOI: https://doi.org/10.1007/BF00585112

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