Abstract
The influence of temperature on the oxygeneration of normal and 2,3-diphosphoglycerate-depleted human blood suspensions (final hemoglobin concentration: 0.75%) was studied under closed-system conditions (constant total CO2 content) beginning with standard values: pH 7.40,P CO 2 40 torr, at 37°C.
The present results quantify the temperature-induced changes inP O 2 occuring in association with the concomitant acid-base variations prevailing in a closed system. When the temperature was raised from 25 to 42°C,P 50 varied from 13.9±1.1 to 40.7±1.9 torr in the presence of 2,3-DPG and from 7.6±0.4 to 24.8±1.2 torr in the absence of the cofactor.
The derived equations correlateP O 2 variations with those of temperature (T: 25–42°C) and oxygen saturation (SO 2: 10–90%).
The temperature coefficient of oxygenation and the DPG-induced decrease in the heat of hemoglobin oxygenation were shown to be saturation dependent. DPG lowered dlogP O 2/dT from 0.0299 to 0.0275 and ΔH from −12.9 to −11.8 kcal/mole O2 bound at 50% SO 2 but had no significant influence on these parameters for SO 2≤20%.
The results suggest that the release of carbamate at the beginning of oxygenation is virtually unaffected by presence of 2,3-DPG in the 25–42°C temperature range.
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Castaing, M., Sinet, M. Temperature and oxygenation of human blood at constant total CO2 content. Pflugers Arch. 386, 135–140 (1980). https://doi.org/10.1007/BF00584200
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DOI: https://doi.org/10.1007/BF00584200