Abstract
The extrusion mechanism ofd-glucose by tubular cells was investigated in isolated rabbit kidneys perfused with normal or hyperglicemic blood, added with14C-inulin, by relating the values of the ratio between the intracellular and extracellular glucose concentration (Gi/Ge) in the cortical tissue to the respective plasma concentration Gp (=Ge). The apparent extracellular space was calculated in each experiment from the kinetics of the14C-inulin efflux from cortical slices excised at the end of the perfusion period (mean value = 20.7%±1.7 of the total vol.). The total intra and extracellular amount of glucose was measured in two contiguous slices excised from the cortex. The total water was expressed by the difference between the fresh and dry weight of the slice, the intracellular water by the difference between the total and extracellular water. From these volumes the respective apparent glucose concentrations could be found. The Gi/Ge values tend to 1, that is to a uniform intra and extracellular distribution of glucose, only at high plasma glucose levels. These results do not seem to be in agreement with ad-glucose transfer from the cell to the subepithelial spaces down a concentration gradient.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barfuss DW, Dantzler WH (1976) Glucose transport in isolated perfused proximal tubules of snake kidney. Am J Physiol 23:1716–1728
Barfuss DW, Schaefer JA (1979) Active amino-acid absorption by proximal convolutes and proximal straight tubules. Am J Physiol 236:F149-F162
Bettge S, Oetliker O (1960) Eine neue Methode zur Bestimmung der Nieren-Clearance am Kaninchen. Naunyn-Schmiedeberg's Arch Exp Pathol Pharmakol 240:187–195
Burg NB, Orloff J (1973) Perfusion of isolated renal tubules. In: Handbook of physiology Section 8, Renal physiology. William and Wilkins Company, Baltimore, p 145
Esposito G, Faelli A, Capraro V (1975) A critical evaluation of the existence of an outward sugar pump in the basolateral membrane of the enterocyte. In: Kramer M, Lauterbach F (eds) Intestinal Permeation. Proceedings of the IV Workshop Conference, Hoechst. Excerpta Medica, Amsterdam, p 107
Esposito G, Faelli A, Capraro V (1976) Effect of ethylacetate on the transport of sodium and glucose in the hamster small intestine in vitro. Biochem Biophys Acta 426:489–498
Frash W, Frohnert PP, Bode F, Baumann J, Kinne R (1970) Competitive inhibition of phloridzin binding byd-glucose and the influence of sodium: a study on isolated brush border membrane rat kidney. Pflügers Arch 320:265–284
Kinne R, Kinne-Saffran E, Murer B (1973) Uptake ofd-glucose by brush border microvilli and membranes from lateral and basal infolding isolated from kidney cortex. Pflügers Arch 343:(Suppl) R46
Kleinzeller A, Kolinská J, Beneš I (1967) Transport of glucose and galactose in kidney-cortex cells. Biochem J 104:843–851
Kleinzeller A (1970) Active sugar transport in renal cortex cells. The electrolyte requirement. Biochem Biophys Acta 211:264–276
Kleinzeller A, Dubyak GR, Griffin P, McAvoy EM, Mullin JM, Rittmaster R (1979) Renal sugar transport in winter flounders. III. Two glucose transport system. Am J Physiol 232:F277–334
Milla E, Zanoli F (1976) Sugar Reabsorption and its relation to sodium transport in isolated rabbit kidney perfused in vitro. Atti Acc Naz dei Lincei LX 2:159–169
Needleman Ph, Passonneau J, Lowry OH (1968) Distribution of glucose and related metabolites in rat kidney. Am J Physiol 215:655–659
Rosemberg LE, Downing SJ, Segal S (1962) Extracellular space estimation in rat kidney slices using14C saccarides and phloridzin. Am J Physiol 202:800–804
Spector WS (1956) Handbook of biological data. W. B. Saunder's Company, Philadelphia and London
Torelli G, Milla E, Faelli A, Costantini S (1966) Energy Requirement for Na+ reabsorption in the in vivo rabbit kidney. Am J Physiol 211:576–580
Torelli G, Milla E, Kleinman L, Faelli A (1973) Effect of hypothermia on renal Na+ reabsorption. Pflügers Arch 342:219–230
Tune BM, Burg MB (1971) Glucose transport by proximal renal tubules. Am J Physiol 221:580–585
Ullrich KJ, Rumrich G, Klöss S (1974) Specificity and sodium dependence of the active sugar transport in the proximal convolution of the rat kidney. Pflügers Arch 351:35–38
Vogel G, Kröger W (1966) Die Bedeutung des Transportes, der Konzentration und der Darbietungsrichtung von Na+ für den tubularen Glucose- und PAH-Transport. Pflügers Arch ges Physiol 288:342–358
Werner W, Rey HG, Wielinger H (1970) Über die Eigenschaften eines neuen Chromogens für die Blutzucker-Bestimmung nach der God-Pod Methode. Fresenius' Z Anal Chem 252:224–230
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Milla, E. Tubular extrusion ofd-glucose in the isolated rabbit kidney perfused in vitro. Pflugers Arch. 388, 29–35 (1980). https://doi.org/10.1007/BF00582625
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00582625