Abstract
An RNA polymerase III transcript, 7SK nuclear RNA, was found to bear sequence homology to the B2 class of highly repeated elements in the mouse genome. Northern blot hybridizations between the small RNAs and two B2 clones showed that only one of them (p49C8) hybridized to 7SK RNA. Both clones, however, hybridized to 4.5SI RNA as well as to a third class of nuclear RNA transcripts around 170 nucleotides long, whose levels were found to be greatly increased upon induction of transformation in a mouse 3T3 cell line transformed with a temperature-sensitive mutant of simian virus 40.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aaronson S. A. & Todaro G. J., 1968. J. Cell Physiol. 72: 141–148.
Alwine J. C., Kemp D. & Stark G., 1977. Proc. Natl. Acad. Sci. USA 74: 5350–5354.
Brockman W. W., 1978. J. Virol. 25: 860–870.
Carey M. F., Singh K., Botchan M. & Cozzarelli N., 1986. Mol. Cell. Biol. 6: 3068–3076.
Chirgwin J. M., Przybyla A. E., MacDonald R. J. & Rutter W. J., 1979. Biochemistry 18: 5294–5299.
Edwards D. R., Parfelt C. L. J. & Denhardt D. T., 1985. Mol. Cell. Biol. 5: 3280–3288.
Grigoryan M. S., Kramerov D. A., Tulchinsky E. M., Revasova E. S. & Lukanidin E. M., 1985. The EMBO J. 4: 2209–2215.
Kramerov D. A., Tillib S. U., Lekakh I. V., Ryskov A. P. & Georgiev G. P., 1985. Biochim. Biophys. Acta 824: 85–98.
Kraus J. P., Williamson C. L., Firgaira F. A., Yang-Feng T. L., Münke M., Francke U. & Rosenberg L. E., 1986. Proc. Natl. Acad. Sci. USA 83: 2047–2051.
Krause, M. O., Kurz, J. & Sohn, U., 1984. In: P. Skehan & S. J. Friedman (eds), Growth, Cancer and the Cell Cycle. The Humana Press Inc. pp. 289–296.
Krause M. O., Kurz J., Lovely J. & MacPherson P., 1986. Cancer Res. 46: 1990–1993.
Krayev A. S., Markusheva T. V., Kramerov D. A., Ryskov A. P., Skryabin K. G., Bayer A. A. & Georgiev G. P., 1982. Nucleic Acids Res. 10: 7461–7475.
Majello B., Mantia G. L., Simeone A., Bonicinelli E. & Lania L., 1985. Nature 314: 457–459.
Reddy R. & Busch H., 1983. Prog. Nucl. Acids Res. 30: 127–161.
Reddy R., Henning D., Subrahmanyam C. S. & Busch H., 1984. J. Biol. Chem. 259: 12263–12270.
Rigby P. W. J., Dieckman M., Rhodes C. & Berg P., 1977. J. Mol. Biol. 113: 237–251.
Ringuette M. J., Gordon K., Szyszko J. & Krause M. O., 1982. Can. J. Biochem. 60: 252–262.
ScottM. R. D., Westphal K. & Rigby P. W. J., 1983. Cell 34: 557–567.
Singh K., Carey M., Saragosti S. & Botchan M., 1985. Nature 314: 553–556.
Sohn U., Szyszko J., Coombs D. & Krause M. O., 1983. Proc. Natl. Acad. Sci. USA 80: 7090–7094.
Ullu E. & Melli M., 1982. Nucl. Acids Res. 10: 2209–2223.
Zieve G., Benecke B.-J. & Penman S., 1976. Biochemistry 16: 4520–4525.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gallant, J., Lovely, J. & Krause, M. Localization of small RNAs hybridizable to a B2 clone in the nuclear fraction of mouse cell lines. Mol Biol Rep 12, 49–53 (1987). https://doi.org/10.1007/BF00580650
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00580650